The distribution characteristics of global blaOXA-carrying Klebsiella pneumoniae.
The distribution characteristics of global blaOXA-carrying Klebsiella pneumoniae.
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全球携带blaOXA的肺炎克雷伯菌的分布特征。
DOI：
10.1186/s12879-023-08156-5
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发表时间：
2023-03-29
影响因子：
3.7
通讯作者：
Cao, Xiaoli
中科院分区：
文献类型：
--
作者：
Meng, Lingning;Liu, Ziyao;Liu, Chang;Li, Chuchu;Shen, Han;Cao, Xiaoli
分析全球肺炎克雷伯菌中blaOXA的分布情况及携带blaOXA的克雷伯菌的特点。肺炎。全株K. pneumoniae的DNA序列，通过RT-PCR软件从NCBI下载。质量检查后，通过与抗性决定簇数据库的注释来调查blaOXA在合格基因组中的分布。基于单核苷酸多态性（single nucleotide polymorphism，SNP）构建blaOXA变异株的系统发育树，探讨这些变异株之间的进化关系。利用MLST（多位点序列类型）网站和blaOXA工具确定这些携带blaOXA的菌株的序列类型（ST）。用perl程序提取样品来源、分离国家、日期和寄主，分析菌株的特征。总共12，356 K。pneumoniae基因组下载，合格11,429个。共检测出4386株携带blaOXA变异株5610个，分属blaOXA-1、blaOXA-2、blaOXA-3、blaOXA-4、blaOXA-5、blaOXA-6、blaOXA-7、blaOXA-8、blaOXA-9、blaOXA-9、blaOXA-10、blaOXA-10、blaOXA-11、blaOXA-11、blaOXA-12、blaOXA-13、blaOXA-14、blaOXA-15、blaOXA-12、blaOXA-13、blaOXA-14、blaOXA-15、blaOXA-15、blaOXA-16、blaOXA-17、blaOXA-17、blaOXA-17、blaOXA-18、blaOXA-19、blaOXA-1（n = 2891，51.5%）和blaOXA-9（n = 969，17.3%）是最常见的blaOXA变体，其次是blaOXA-48（n = 800，14.3%）和blaOXA-232（n = 480，8.6%）。系统发育树显示8个分支，其中3个分支由碳青霉烯类苯唑西林酶（CHO）组成。在4386株菌株中共鉴定出300株不同的ST，其中ST 11（n = 477，10.9%）是最主要的ST，其次是ST 258（n = 410，9.4%）。人（2696/4386，61.5%）是携带blaOXA的克雷伯氏菌的主要宿主。肺炎分离株。携带blaOXA-9的K. pneumoniae菌株主要发现于美国，携带blaOXA-48的K.肺炎菌主要分布在欧洲和亚洲。在全球K.在肺炎链球菌中，鉴定出许多blaOXA变体，其中blaOXA-1、blaOXA-9、blaOXA-48和blaOXA-232是最普遍的变体，表明blaOXA在抗微生物剂的选择性压力下迅速进化。ST 11和ST 258是携带blaOXA的主要克隆。肺炎。 在线版本包含补充材料，可通过10.1186/s12879 - 023 - 08156 - 5获得。
To analyze the distribution of blaOXA among global Klebsiella pneumoniae and the characteristics of blaOXA-carrying K. pneumoniae. The genomes of global K. pneumoniae were downloaded from NCBI by Aspera software. After quality check, the distribution of blaOXA among the qualified genomes was investigated by annotation with the resistant determinant database. The phylogenetic tree was constructed for the blaOXA variants based on the single nucleotide polymorphism (SNP) to explore the evolutionary relationship between these variants. The MLST (multi-locus sequence type) website and blastn tools were utilized to determine the sequence types (STs) of these blaOXA-carrying strains. and sample resource, isolation country, date and host were extracted by perl program for analyzing the characteristics of these strains. A total of 12,356 K. pneumoniae genomes were downloaded and 11,429 ones were qualified. Among them, 4386 strains were found to carry 5610 blaOXA variants which belonged to 27 varieties of blaOXAs, blaOXA-1 (n = 2891, 51.5%) and blaOXA-9 (n = 969, 17.3%) were the most prevalent blaOXA variants, followed by blaOXA-48 (n = 800, 14.3%) and blaOXA-232 (n = 480, 8.6%). The phylogenetic tree displayed 8 clades, three of them were composed of carbapenem-hydrolyzing oxacillinase (CHO). Totally, 300 distinct STs were identified among 4386 strains with ST11 (n = 477, 10.9%) being the most predominant one followed by ST258 (n = 410, 9.4%). Homo sapiens (2696/4386, 61.5%) was the main host for blaOXA-carrying K. pneumoniae isolates. The blaOXA-9-carrying K. pneumoniae strains were mostly found in the United States and blaOXA-48-carrying K. pneumoniae strains were mainly distributed in Europe and Asia. Among the global K. pneumoniae, numerous blaOXA variants were identified with blaOXA-1, blaOXA-9, blaOXA-48 and blaOXA-232 being the most prevalent ones, indicating that blaOXA rapidly evolved under the selective pressure of antimicrobial agents. ST11 and ST258 were the main clones for blaOXA-carrying K. pneumoniae. The online version contains supplementary material available at 10.1186/s12879-023-08156-5.
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