Direct on-swab metabolic profiling of vaginal microbiome host interactions during pregnancy and preterm birth.
Direct on-swab metabolic profiling of vaginal microbiome host interactions during pregnancy and preterm birth.
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孕期和早产期间阴道微生物组与宿主相互作用的直接拭子代谢谱分析
DOI：
10.1038/s41467-021-26215-w
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发表时间：
2021-10-13
影响因子：
16.6
通讯作者：
MacIntyre DA
中科院分区：
文献类型：
--
作者：
Pruski P;Correia GDS;Lewis HV;Capuccini K;Inglese P;Chan D;Brown RG;Kindinger L;Lee YS;Smith A;Marchesi J;McDonald JAK;Cameron S;Alexander-Hardiman K;David AL;Stock SJ;Norman JE;Terzidou V;Teoh TG;Sykes L;Bennett PR;Takats Z;MacIntyre DA
妊娠期阴道微生物群增加了早产风险，早产是5岁以下儿童死亡的主要原因。在这里，我们通过解吸电喷雾电离质谱法（DESI-MS）对两个独立妊娠队列（VMET, n = 160； 455拭子；VMET II, n = 205； 573拭子）的宫颈阴道代谢组进行了直接的拭子代谢谱分析。通过整合来自匹配样本的元组学和免疫谱数据，我们发现特定的代谢组特征可用于同时预测阴道微生物组的组成和宿主炎症状态。在这些患者中，如DESI-MS预测的那样，阴道微生物群不稳定和先天免疫激活与早产有关，包括接受宫颈环扎术预防早产的妇女。这些发现强调了DESI-MS作为一种通过快速评估阴道微生物群-宿主动态来进行早产风险分层的创新方法。在这里，作者应用DESI-MS（一种无需样品制备的直接棉签质谱分析工具）来分析两个独立队列的孕妇的宫颈阴道代谢组，并结合匹配的元分类和免疫分析数据，表明DESI-MS预测阴道微生物群组成和局部炎症状态与早产和妊娠期间使用的临床干预措施相关。
The pregnancy vaginal microbiome contributes to risk of preterm birth, the primary cause of death in children under 5 years of age. Here we describe direct on-swab metabolic profiling by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) for sample preparation-free characterisation of the cervicovaginal metabolome in two independent pregnancy cohorts (VMET, n = 160; 455 swabs; VMET II, n = 205; 573 swabs). By integrating metataxonomics and immune profiling data from matched samples, we show that specific metabolome signatures can be used to robustly predict simultaneously both the composition of the vaginal microbiome and host inflammatory status. In these patients, vaginal microbiota instability and innate immune activation, as predicted using DESI-MS, associated with preterm birth, including in women receiving cervical cerclage for preterm birth prevention. These findings highlight direct on-swab metabolic profiling by DESI-MS as an innovative approach for preterm birth risk stratification through rapid assessment of vaginal microbiota-host dynamics. Here, the authors apply DESI-MS, a sample preparation-free, direct on-swab mass spectrometry analytical tool, to profile the cervicovaginal metabolome of two independent cohorts of pregnant women and, combined with matched metataxonomic and immuno-profiling data, show that DESI-MS predicts vaginal microbiota composition and local inflammatory status associated with preterm birth and clinical interventions used during pregnancy.
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阴道营养不良增加了早产胎儿膜破裂，新生儿败血症的风险，并因红霉素而加剧。
影响因子：
9.3
作者：
Brown RG;Marchesi JR;Lee YS;Smith A;Lehne B;Kindinger LM;Terzidou V;Holmes E;Nicholson JK;Bennett PR;MacIntyre DA
通讯作者：
MacIntyre DA
影响因子：
4.4
作者：
Fettweis JM;Serrano MG;Sheth NU;Mayer CM;Glascock AL;Brooks JP;Jefferson KK;Vaginal Microbiome Consortium (additional members);Buck GA
通讯作者：
Buck GA
DOI：
10.1073/pnas.1502875112
发表时间：
2015-09-01
影响因子：
11.1
作者：
DiGiulio, Daniel B.;Callahan, Benjamin J.;Relman, David A.
通讯作者：
Relman, David A.
DOI：
10.1111/1471-0528.15981
发表时间：
2019-11-20
影响因子：
5.8
作者：
Borgogna, J. C.;Shardell, M. D.;Brotman, R. M.
通讯作者：
Brotman, R. M.
影响因子：
3
作者：
Al-Mushrif, S;Eley, A;Jones, BM
通讯作者：
Jones, BM