Intraductal injection for localized drug delivery to the mouse mammary gland.
Intraductal injection for localized drug delivery to the mouse mammary gland.
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DOI：
10.3791/50692
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发表时间：
2013-10-04
期刊：
影响因子：
--
通讯作者：
Ingber DE
中科院分区：
文献类型：
--
作者：
Krause S;Brock A;Ingber DE
在这里，我们描述了一种通过导管内注射将各种试剂输送到小鼠乳腺的方案。由于缺乏独立于发育阶段（如妊娠期和哺乳期）的合适靶向分子，很难实现乳腺上皮内的局部药物递送和基因敲除。在此，我们描述了一种通过导管内注射到小鼠乳头，在成年期任何阶段将试剂局部递送到乳腺的技术。这种注射可以在麻醉下对活体小鼠进行，并允许非侵入性和局部药物递送到乳腺。此外，注射可以在几个月内重复进行，而不会损伤乳头。重要的染料，如埃文斯蓝是非常有助于学习技术。在导管内注射蓝色染料后，眼睛可以看到整个导管树。此外，荧光标记试剂也允许可视化和乳腺内的分布。该技术适用于各种化合物，包括siRNA，化疗药物和小分子。
Herein we describe a protocol to deliver various reagents to the mouse mammary gland via intraductal injections. Localized drug delivery and knock-down of genes within the mammary epithelium has been difficult to achieve due to the lack of appropriate targeting molecules that are independent of developmental stages such as pregnancy and lactation. Herein, we describe a technique for localized delivery of reagents to the mammary gland at any stage in adulthood via intraductal injection into the nipples of mice. The injections can be performed on live mice, under anesthesia, and allow for a non-invasive and localized drug delivery to the mammary gland. Furthermore, the injections can be repeated over several months without damaging the nipple. Vital dyes such as Evans Blue are very helpful to learn the technique. Upon intraductal injection of the blue dye, the entire ductal tree becomes visible to the eye. Furthermore, fluorescently labeled reagents also allow for visualization and distribution within the mammary gland. This technique is adaptable for a variety of compounds including siRNA, chemotherapeutic agents, and small molecules.
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影响因子：
0.9
作者：
Flanagan, Meghan;Love, Susan;Hwang, E Shelley
通讯作者：
Hwang, E Shelley
DOI：
10.1186/bcr2358
发表时间：
2009
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Behbod F;Kittrell FS;LaMarca H;Edwards D;Kerbawy S;Heestand JC;Young E;Mukhopadhyay P;Yeh HW;Allred DC;Hu M;Polyak K;Rosen JM;Medina D
通讯作者：
Medina D
DOI：
10.1186/bcr1410
发表时间：
2006
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
King BL;Love SM
通讯作者：
Love SM
影响因子：
17.1
作者：
Stearns V;Mori T;Jacobs LK;Khouri NF;Gabrielson E;Yoshida T;Kominsky SL;Huso DL;Jeter S;Powers P;Tarpinian K;Brown RJ;Lange JR;Rudek MA;Zhang Z;Tsangaris TN;Sukumar S
通讯作者：
Sukumar S
DOI：
10.1093/jnci/93.21.1624
发表时间：
2001-11-07
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
Dooley, WC;Ljung, BM;King, EB
通讯作者：
King, EB