Immunohistochemical Localization of MD2, a Co-Receptor of TLR4, in the Adult Mouse Brain.
Immunohistochemical Localization of MD2, a Co-Receptor of TLR4, in the Adult Mouse Brain.
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DOI：
10.1021/acschemneuro.2c00540
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发表时间：
2023-02-01
影响因子：
5
通讯作者：
Liang, Huazheng
中科院分区：
文献类型：
--
作者：
Li, Zhen;Chen, Aiwen;Wan, Hanxi;Gao, Xiaofei;Li, Chunguang;Xiong, Lize;Liang, Huazheng
关键词：
髓系分化因子2(MD2)是一种经典的促炎蛋白TLR4的辅受体，其激活可导致神经炎症。人们普遍认为TLR4表达于小胶质细胞和星形胶质细胞的细胞表面，MD2也有望在这些细胞中表达。然而，我们先前的研究表明，某些核团的神经元也表达MD2。MD2是否由其他大脑核团表达仍是个未知数。本研究的目的是定位MD2阳性细胞在成年小鼠脑内的分布。通过与小鼠脑图谱比较MD2阳性细胞的位置，完成MD2免疫组织化学染色，以定位MD2阳性细胞。MD2阳性细胞见于大多数小鼠脑核，嗅球、大脑皮层、红核和颅核有成团的细胞团。皮质下核团MD2染色不均，基底外侧核和中央杏仁核细胞更为明显。腹侧苍白球和斜角带内有密度和形状相似的阳性细胞。丘脑核团中有突出的细胞，细胞密度相近，分布在脑干的网状结构中。与丘脑相比，下丘脑的突出细胞较少。顶盖前的红核、黑质和腹侧被盖区有突出的细胞。运动颅核也有MD2阳性细胞，而中缝、感觉颅核和小脑深部核有中等密度的MD2阳性细胞。MD2在这些核团中的存在可能提示MD2参与了相应的生理功能。
Myeloid differentiation factor 2 (MD2) is a co-receptor of a classical proinflammatory protein TLR4 whose activation leads to neuroinflammation. It is widely accepted that TLR4 is expressed on the cell surface of microglia and astrocytes, and MD2 is expected to be expressed by these cells as well. However, our previous study showed that neurons from certain nuclei also expressed MD2. Whether MD2 is expressed by other brain nuclei is still unknown. It is the aim of the present study to map the distribution of MD2-positive cells in the adult mouse brain. Immunohistochemical staining against MD2 was completed to localize MD2-positive cells in the mouse brain by comparing the location of positive cells with the mouse brain atlas. MD2-positive cells were found in the majority of mouse brain nuclei with clusters of cells in the olfactory bulb, cortices, the red nucleus, and cranial nuclei. Subcortical nuclei had heterogeneous staining of MD2 with more prominent cells in the basolateral and the central amygdaloid nuclei. The ventral pallidum and the diagonal bands had positive cells with similar density and shape. Prominent cells were present in thalamic nuclei which were nearly homogeneous and in reticular formation of the brainstem where cells were dispersed with similar density. The hypothalamus had fewer outstanding cells compared with the thalamus. The red nucleus, the substantia nigra, and the ventral tegmental area in the pretectum had outstanding cells. Motor cranial nuclei also had outstanding MD2-positive cells, whereas raphe, sensory cranial, and deep cerebellar nuclei had MD2-positive cells with moderate density. The presence of MD2 in these nuclei may suggest the involvement of MD2 in their corresponding physiological functions.
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