Immediate pools of malaria infections at diagnosis combined with targeted deep sequencing accurately quantifies frequency of drug resistance mutations.
Immediate pools of malaria infections at diagnosis combined with targeted deep sequencing accurately quantifies frequency of drug resistance mutations.
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作者：
Aydemir O;Mensah B;Marsh PW;Abuaku B;Myers-Hansen JL;Bailey JA;Ghansah A
撒哈拉以南非洲地区的抗疟药耐药性监测往往受到后勤和财务挑战的限制，限制了监测的广度和频率。在加纳的两个地点，我们试行了一个简化的样本池流程，该流程是通过在诊断测试时顺序添加疟疾阳性病例而立即创建的。这种涉及单管的简化流程最大限度地减少了临床和实验室工作，并在使用分子倒置探针进行高通量靶向测序后提供了所有已知耐药突变的准确频率。我们的研究验证了这种方法是一种经济有效、准确且高度可扩展的耐药突变监测方法，有可能应用于结核病等其他传染病。
Antimalarial resistance surveillance in sub-Saharan Africa is often constrained by logistical and financial challenges limiting its breadth and frequency. At two sites in Ghana, we have piloted a streamlined sample pooling process created immediately by sequential addition of positive malaria cases at the time of diagnostic testing. This streamlined process involving a single tube minimized clinical and laboratory work and provided accurate frequencies of all known drug resistance mutations after high-throughput targeted sequencing using molecular inversion probes. Our study validates this method as a cost-efficient, accurate and highly-scalable approach for drug resistance mutation monitoring that can potentially be applied to other infectious diseases such as tuberculosis.
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DOI：
10.1093/infdis/jiy223
发表时间：
2018-08-14
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Aydemir O;Janko M;Hathaway NJ;Verity R;Mwandagalirwa MK;Tshefu AK;Tessema SK;Marsh PW;Tran A;Reimonn T;Ghani AC;Ghansah A;Juliano JJ;Greenhouse BR;Emch M;Meshnick SR;Bailey JA
通讯作者：
Bailey JA
影响因子：
9.3
作者：
Wesolowski A;Taylor AR;Chang HH;Verity R;Tessema S;Bailey JA;Alex Perkins T;Neafsey DE;Greenhouse B;Buckee CO
通讯作者：
Buckee CO
影响因子：
10.7
作者：
Cheeseman IH;McDew-White M;Phyo AP;Sriprawat K;Nosten F;Anderson TJ
通讯作者：
Anderson TJ
DOI：
10.4269/ajtmh.15-0007
发表时间：
2015-09
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
Cui L;Mharakurwa S;Ndiaye D;Rathod PK;Rosenthal PJ
通讯作者：
Rosenthal PJ
影响因子：
14.9
作者：
Hathaway NJ;Parobek CM;Juliano JJ;Bailey JA
通讯作者：
Bailey JA