Plasmodium vivax sporozoite challenge in malaria-naïve and semi-immune Colombian volunteers.
Plasmodium vivax sporozoite challenge in malaria-naïve and semi-immune Colombian volunteers.
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DOI：
10.1371/journal.pone.0099754
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Herrera S
中科院分区：
文献类型：
--
作者：
Arévalo-Herrera M;Forero-Peña DA;Rubiano K;Gómez-Hincapie J;Martínez NL;Lopez-Perez M;Castellanos A;Céspedes N;Palacios R;Oñate JM;Herrera S
最近在研究人类间日疟原虫挑战感染方面取得了重大进展，这对疫苗和药物测试至关重要。为了加速疟疾疫苗的临床开发，比较了naïve和半免疫志愿者因受感染蚊子叮咬而实验性感染的结果。7只malaria-naïve和9只半免疫的哥伦比亚成年蚊（n = 16）被2-4只感染间日疟原虫孢子虫的按蚊叮咬。评估和比较寄生虫血症水平、疟疾临床表现和免疫反应。所有志愿者均经显微镜和RT-qPCR证实感染。在专利前期间（malaria-naïve和疟疾暴露者的平均时间分别为12.5天和12.8天）没有观察到显著差异，但naïve志愿者出现了典型的疟疾体征和症状，而半免疫志愿者在诊断之日表现出轻微或没有症状。一位malaria-naïve志愿者患上了一种短暂的低亚显微寄生虫病，这种病可以自行治愈。感染诱导两组特异性抗体水平升高。孢子虫感染在半免疫和naïve志愿者中是安全且可重复的。该模型将为在控制条件下同时比较naïve和半免疫志愿者的间日疟原虫疫苗的保护效果提供信息，并将加速间日疟原虫疫苗的开发。clinicaltrials.gov NCT01585077
Significant progress has been recently achieved in the development of Plasmodium vivax challenge infections in humans, which are essential for vaccine and drug testing. With the goal of accelerating clinical development of malaria vaccines, the outcome of infections experimentally induced in naïve and semi-immune volunteers by infected mosquito bites was compared. Seven malaria-naïve and nine semi-immune Colombian adults (n = 16) were subjected to the bites of 2–4 P. vivax sporozoite-infected Anopheles mosquitoes. Parasitemia levels, malaria clinical manifestations, and immune responses were assessed and compared. All volunteers developed infections as confirmed by microscopy and RT-qPCR. No significant difference in the pre-patent period (mean 12.5 and 12.8 days for malaria-naïve and malaria-exposed, respectively) was observed but naïve volunteers developed classical malaria signs and symptoms, while semi-immune volunteers displayed minor or no symptoms at the day of diagnosis. A malaria-naïve volunteer developed a transient low submicroscopic parasitemia that cured spontaneously. Infection induced an increase in specific antibody levels in both groups. Sporozoite infectious challenge was safe and reproducible in semi-immune and naïve volunteers. This model will provide information for simultaneous comparison of the protective efficacy of P. vivax vaccines in naïve and semi-immune volunteers under controlled conditions and would accelerate P. vivax vaccine development. clinicaltrials.gov NCT01585077
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影响因子：
--
作者：
Arevalo-Herrera, M;Roggero, MA;Herrera, S
通讯作者：
Herrera, S
影响因子：
3
作者：
Chaparro P;Padilla J;Vallejo AF;Herrera S
通讯作者：
Herrera S
影响因子：
1.9
作者：
BEIER, JC;ONYANGO, FK;ROBERTS, CR
通讯作者：
ROBERTS, CR
DOI：
10.2987/8756-971x(2005)21
发表时间：
2005-09-01
影响因子：
1
作者：
Grieco, JP;Achee, NL;Andre, RG
通讯作者：
Andre, RG
DOI：
10.4269/ajtmh.2011.09-0516
发表时间：
2011-02-01
影响因子：
3.3
作者：
Herrera, Socrates;Lucia Fernandez, Olga;Arevalo-Herrera, Myriam
通讯作者：
Arevalo-Herrera, Myriam