Expression of autophagy-related protein Beclin-1 in malignant canine mammary tumors.
Expression of autophagy-related protein Beclin-1 in malignant canine mammary tumors.
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DOI：
10.1186/1746-6148-9-75
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发表时间：
2013-04-11
影响因子：
2.6
通讯作者：
Liu CH
中科院分区：
文献类型：
--
作者：
Liu JL;Chang KC;Lo CC;Chu PY;Liu CH
自噬是一种通过溶酶体酶降解不必要的细胞成分的自我分解代谢机制。Beclin-1是一种自噬相关蛋白，首次建立了自噬与肿瘤发生之间的联系。本研究的目的是评估Beclin-1的表达模式，并确定其预后意义的恶性犬乳腺肿瘤（CMT）患者。我们用免疫组化方法检测了70例恶性CMT中Beclin-1的表达。胞浆Beclin-1在癌细胞中的表达明显弱于邻近的正常乳腺（p < 0.001）。低胞浆表达（57.14%）与年龄较大、肾小管形成程度较低、有丝分裂活性增加、组织学分级较高和广泛坏死有关。低核表达（40%）与年龄较大、肾小管形成程度较低、广泛坏死和Her 2/neu过表达阴性有关。单因素生存分析显示Beclin-1胞浆表达是影响总生存率的不良预后因素（p < 0.001）。多变量生存分析表明，Beclin-1细胞质表达是一个独立的预后因素（p = 0.016）。Beclin-1的缺失与侵袭性临床病理特征和较差的总体存活率相关。结果表明，Beclin-1在恶性CMT的肿瘤进展中起重要作用。
Autophagy is a self-catabolic mechanism that degrades unnecessary cellular components through lysosomal enzymes. Beclin-1, an autophagy-related protein, establishes the first connection between autophagy and tumorigenesis. The purpose of this study is to assess the Beclin-1 expression pattern and to determine its prognostic significance in patients with malignant canine mammary tumor (CMT). We examined Beclin-1 expression in 70 cases of malignant CMTs by immunohistochemistry. Cytoplasmic Beclin-1 expression was significantly weaker in cancer cells than in nearby normal mammary glands (p < 0.001). Low cytoplasmic expression (57.14%) was associated with older age, lower degree of tubular formation, increased mitotic activity, higher histologic grade, and extensive necrosis. Low nuclear expression (40%) was connected with older age, lower degree of tubular formation, extensive necrosis, and negative for Her2/neu overexpression. Univariate survival analysis showed that Beclin-1 cytoplasmic expression was a poor prognostic factor for overall survival rate (p < 0.001). Multivariate survival analysis demonstrated that Beclin-1 cytoplasmic expression is an independent prognostic factor (p = 0.016). Loss of Beclin-1 is associated with aggressive clinicopathologic features and poor overall survival. The results suggest that Beclin-1 plays an important role in tumor progression of malignant CMTs.
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影响因子：
158.5
作者：
Krueger, Darcy A.;Care, Marguerite M.;Franz, David Neal
通讯作者：
Franz, David Neal
影响因子：
3.3
作者：
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通讯作者：
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影响因子：
4.3
作者：
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通讯作者：
Lisanti, Michael P.
影响因子：
2.8
作者：
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通讯作者：
Liu, Rong-Yu
影响因子：
56.9
作者：
Kirkness, EF;Bafna, V;Venter, JC
通讯作者：
Venter, JC