The interaction of force and repetition on musculoskeletal and neural tissue responses and sensorimotor behavior in a rat model of work-related musculoskeletal disorders.
The interaction of force and repetition on musculoskeletal and neural tissue responses and sensorimotor behavior in a rat model of work-related musculoskeletal disorders.
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DOI：
10.1186/1471-2474-14-303
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发表时间：
2013-10-25
影响因子：
2.3
通讯作者：
Barr-Gillespie AE
中科院分区：
文献类型：
--
作者：
Barbe MF;Gallagher S;Massicotte VS;Tytell M;Popoff SN;Barr-Gillespie AE
我们检查了肌肉骨骼危险因素，潜在的力量和重复对组织反应的关系，在一个重复性伸手和牵拉的操作大鼠模型中，如果力量x重复相互作用存在，表明疲劳失效过程。我们研究了连续12周在四种重复和力量水平下重复拉手柄任务时发生的生化、形态学和感觉运动反应的暴露依赖性变化：1)低强度重复，2)低强度重复，3)低强度重复，4)高强度重复（HRHF）。大鼠进行4-6周的初始训练，然后连续12周，每天2小时，每周3天执行其中一项任务。反射性握力和触觉敏感性作为功能指标进行分析。采用ELISA法检测指屈肌和肌腱、前肢骨和血清的炎症、组织应激和修复以及骨转换指标。组织形态测定法检测组织巨噬细胞浸润、脊髓P物质变化及组织适应性或降解性变化。MicroCT用于测定骨质量的变化。几个力x重复相互作用观察到：肌肉il -1 α和骨il -1 β；血清TNFalpha、il -1 α和il -1 β；肌肉HSP72，一种组织应激和修复蛋白；肌腱和软骨退化的组织形态学证据；骨降解（CTXI）和骨形成（骨钙素）的血清生物标志物；以及骨适应和骨吸收的形态学证据。在大多数情况下，HRHF任务的表现诱导了最大的组织退行性改变，而中等水平任务的表现诱导了骨适应和肌肉适应。在任务第12周时，两项高强度任务均引起正中神经巨噬细胞浸润、脊髓致敏（P物质增加）、握力下降和前爪机械异常痛。虽然在所有组织中并不一致，但我们发现在力和重复的关键肌肉骨骼危险因素之间有几个显著的相互作用，与肌肉骨骼组织的疲劳失效过程一致。HRHF任务的长时间表现出肌肉骨骼疾病的风险显著增加，而中等水平任务的表现表现出对任务需求的适应。
We examined the relationship of musculoskeletal risk factors underlying force and repetition on tissue responses in an operant rat model of repetitive reaching and pulling, and if force x repetition interactions were present, indicative of a fatigue failure process. We examined exposure-dependent changes in biochemical, morphological and sensorimotor responses occurring with repeated performance of a handle-pulling task for 12 weeks at one of four repetition and force levels: 1) low repetition with low force, 2) high repetition with low force, 3) low repetition with high force, and 4) high repetition with high force (HRHF). Rats underwent initial training for 4–6 weeks, and then performed one of the tasks for 12 weeks, 2 hours/day, 3 days/week. Reflexive grip strength and sensitivity to touch were assayed as functional outcomes. Flexor digitorum muscles and tendons, forelimb bones, and serum were assayed using ELISA for indicators of inflammation, tissue stress and repair, and bone turnover. Histomorphometry was used to assay macrophage infiltration of tissues, spinal cord substance P changes, and tissue adaptative or degradative changes. MicroCT was used to assay bones for changes in bone quality. Several force x repetition interactions were observed for: muscle IL-1alpha and bone IL-1beta; serum TNFalpha, IL-1alpha, and IL-1beta; muscle HSP72, a tissue stress and repair protein; histomorphological evidence of tendon and cartilage degradation; serum biomarkers of bone degradation (CTXI) and bone formation (osteocalcin); and morphological evidence of bone adaptation versus resorption. In most cases, performance of the HRHF task induced the greatest tissue degenerative changes, while performance of moderate level tasks induced bone adaptation and a suggestion of muscle adaptation. Both high force tasks induced median nerve macrophage infiltration, spinal cord sensitization (increased substance P), grip strength declines and forepaw mechanical allodynia by task week 12. Although not consistent in all tissues, we found several significant interactions between the critical musculoskeletal risk factors of force and repetition, consistent with a fatigue failure process in musculoskeletal tissues. Prolonged performance of HRHF tasks exhibited significantly increased risk for musculoskeletal disorders, while performance of moderate level tasks exhibited adaptation to task demands.
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影响因子：
1.8
作者：
Andersen, Lars L.;Holtermann, Andreas;Sjogaard, Gisela
通讯作者：
Sjogaard, Gisela
影响因子：
3.3
作者：
BOURRIN, S;GENTY, C;ALEXANDRE, C
通讯作者：
ALEXANDRE, C
影响因子：
5.5
作者：
Brooks, SV;Faulkner, JA
通讯作者：
Faulkner, JA
影响因子：
6.2
作者：
Barr, AE;Safadi, FF;Barbe, MF
通讯作者：
Barbe, MF
影响因子：
1.8
作者：
Asundi, Krishna R.;Rempel, David M.
通讯作者：
Rempel, David M.