Fatty acid-mediated endoplasmic reticulum stress in vivo: differential response to the infusion of Soybean and Lard Oil in rats.
Fatty acid-mediated endoplasmic reticulum stress in vivo: differential response to the infusion of Soybean and Lard Oil in rats.
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DOI：
10.1016/j.metabol.2012.12.001
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发表时间：
2013-05
影响因子：
9.8
通讯作者：
Pagliassotti, Michael J.
中科院分区：
文献类型：
--
作者：
Nivala, Angela M.;Reese, Lauren;Frye, Melinda;Gentile, Christopher L.;Pagliassotti, Michael J.
在细胞系统中，与不饱和脂肪酸相比，饱和脂肪酸会在许多细胞类型（包括肝细胞和脂肪细胞）中诱导更大程度的内质网应激和炎症信号传导。本研究的目的是确定输注猪油（富含饱和脂肪酸）和大豆油（富含不饱和脂肪酸）对肝脏和脂肪组织内质网应激和体内炎症信号传导的影响。将含有甘油、磷脂酰胆碱、抗生素（对照，n=7）和大豆油（Soybean，n=7）或猪油（Lard，n=7）的脂质乳液在4小时内静脉内输注给大鼠。对照组的血浆游离脂肪酸水平为 0.5 ± 0.1 mM（平均值 ± 标准差），大豆和猪油的血浆游离脂肪酸水平分别增加至 1.0 ± 0.3 mM 和 1.1 ± 0.3 mM。各组之间的血糖和胰岛素水平没有差异。与对照相比，大豆和猪油的肝脏和脂肪组织中内质网（ER）应激和炎症通路信号激活的标记物增加，但与大豆相比，猪油的增加程度更大。这些数据表明，升高的血浆游离脂肪酸可以诱导肝脏和脂肪组织内质网应激和体内炎症。此外，饱和脂肪酸在体内似乎比不饱和脂肪酸更具细胞毒性。
In cell systems, saturated fatty acids, compared to unsaturated fatty acids,induce a greater degree of ER stress and inflammatory signaling in a number of cell types, including hepatocytes and adipocytes. The aim of the present study was to determine the effects of infusions of lard oil (enriched in saturated fatty acids) and soybean oil (enriched in unsaturated fatty acids) on liver and adipose tissue ER stress and inflammatory signaling in vivo. Lipid emulsions containing glycerol, phosphatidylcholine, antibiotics (Control, n=7) and either soybean oil (Soybean, n=7) or lard oil (Lard, n=7) were infused intravenously into rats over a 4 hour period. Plasma free fatty acid levels were 0.5 ± 0.1 mM (mean ± SD) in Control and were increased to 1.0 ± 0.3 mM and 1.1 ± 0.3 mM in Soybean and Lard, respectively. Glucose and insulin levels were not different among groups. Markers of endoplasmic reticulum (ER) stress and activation of inflammatory pathway signaling were increased in liver and adipose tissue from Soybean and Lard compared to Control, but were increased to a greater extent in Lard compared to Soybean. These data suggest that elevated plasma free fatty acids can induce hepatic and adipose tissue ER stress and inflammation in vivo. In addition, saturated fatty acids appear to be more cytotoxic than unsaturated fatty acids in vivo.
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影响因子：
4.8
作者：
Li, Zheng Zheng;Berk, Michael;Feldstein, Ariel E.
通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.1101379108
发表时间：
2011-05-10
影响因子：
11.1
作者：
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影响因子：
3.1
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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