A rice PHD-finger protein OsTITANIA, is a growth regulator that functions through elevating expression of transporter genes for multiple metals.
A rice PHD-finger protein OsTITANIA, is a growth regulator that functions through elevating expression of transporter genes for multiple metals.
复制标题
水稻 PHD 指蛋白 OsTITANIA 是一种生长调节剂，通过提高多种金属转运蛋白基因的表达来发挥作用。
作者：
Tanaka;N.;Uraguchi;S.;Kajikawa;M.;Saito;A.;Ohmori;Y.;Fujiwara;T.
植物生长对必需金属的吸收主要由金属特异性转运蛋白介导，其表达对特定金属的环境或细胞条件作出反应。与金属特异性调控不同，我们描述了一种组成型表达的转录因子，它调控水稻中几种金属的运输。我们表征了水稻突变体低镉5（LC 5），其表现出生长和必需金属（例如，铜（Cu）、锌（Zn）和锰（Mn）。LC 5矮秆，分蘖少，但维管结构明显正常。分子遗传学分析表明，LC 5的致病基因是转录调节因子拟南芥TITANIA（TTA）的直系同源物。表达分析表明，OsTTA基因编码一个核定位蛋白，含有植物同源结构域-指（PHD-finger）结构域，在水稻中广泛表达。RNA测序和定量PCR分析表明，转运基因的mRNA积累的必需金属，包括铁（Fe），锌，锰，大幅低于LC 5根比野生型。与已知的金属转运调控转录因子不同，OsTTA转录本的积累不受金属有效性的影响。此外，LC 5的生长缺陷被部分拯救的Fe，Zn，或Mn补充，分别。总的来说，OsTTA是多个金属转运蛋白基因的组成型表达调节因子，负责将必需金属递送到芽以使其正常生长。
Essential metal absorption for plant growth is mediated predominantly by metal‐specific transporters, with expression that responds to the environmental or cellular conditions of specific metals. Differing from metal‐specific regulation, we describe a constitutively expressed transcription factor that regulates the transport of several metals in rice. We characterized the rice mutant LOW CADMIUM 5 (LC5), which exhibited reduced growth and accumulation of essential metals (e.g., copper [Cu], zinc [Zn] and manganese [Mn]) in shoots. LC5 was dwarf and developed less tillers than the wild type, but the structure of vasculature was apparently normal. Molecular genetic analysis revealed that the causal gene of LC5 is an ortholog of the transcriptional regulatorArabidopsis thaliana TITANIA(TTA), known as a transcriptional regulator. Expression analyses demonstrated that theOsTTAgene encodes a nucleus‐localized protein containing a plant homeodomain‐finger (PHD‐finger) domain and is expressed ubiquitously in rice plants. RNA sequencing and quantitative PCR analyses revealed that the mRNA accumulation of transporter genes for essential metals, including iron (Fe), Zn, or Mn, were substantially lower in LC5 roots than in the wild type. Unlike known transcription factors of metal transport regulation,OsTTAtranscript accumulation was not affected by metal availability. In addition, the growth defect of LC5 was partially rescued by Fe, Zn, or Mn supplementation, respectively. Taken together, OsTTA is a constitutively expressed regulator of multiple metal transporter genes responsible for essential metals delivery to shoots for their normal growth.
登录
查看更多内容
影响因子：
6.4
作者：
Kobayashi, T;Yoshihara, T;Nishizawa, NK
通讯作者：
Nishizawa, NK
影响因子：
7.3
作者：
Arnold, Tim;Kirk, Guy J. D.;Weiss, Dominik J.
通讯作者：
Weiss, Dominik J.
拟南芥 MALE MEIOCYTE DEATH1 基因编码雄性减数分裂所需的 PHD 指蛋白。
DOI：
--
发表时间：
2003
期刊：
The Plant cell
影响因子：
--
作者：
Yang,Xiaohui;Makaroff,ChristopherA;Ma,Hong
通讯作者：
Ma,Hong
影响因子：
6.9
作者：
Takahashi R;Ishimaru Y;Senoura T;Shimo H;Ishikawa S;Arao T;Nakanishi H;Nishizawa NK
通讯作者：
Nishizawa NK
影响因子：
3
作者：
WELCH, WJ
通讯作者：
WELCH, WJ