A new apparatus to analyze meal-related ingestive behaviors in rats fed a complex multi-food diet.
A new apparatus to analyze meal-related ingestive behaviors in rats fed a complex multi-food diet.
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DOI：
10.1016/j.physbeh.2022.113824
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发表时间：
2022-08-01
影响因子：
2.9
通讯作者：
Spector AC
中科院分区：
文献类型：
--
作者：
Blonde GD;Fletcher FH;Tang T;Newsome R;Spector AC
测量进餐的大小和时间提供了对食物摄入过程的重要见解。虽然大多数工作都是用单一的食物或液体进行的，但食物选择的可用性也会影响饮食并与这些过程相互作用。5项食物选择监测器（FCM）是一种连续测量大鼠进食和饮水行为的装置，同时提供多达5种食物和2种液体，旨在研究食物选择与膳食模式的同时进行。为了验证该装置，将成年雄性和雌性（各n=8）Sprague-Dawley大鼠饲养在FCM中。连续测量食物和液体摄入量（22小时/天），同时向大鼠提供水和粉末状食物。然后提供5种在大量营养素含量、质地和风味上不同的食物的自助餐饮食沿着水。最后，将5种食物与0.3 M蔗糖和水一起沿着。进行分析，以找到最佳的标准，将摄食行为打包到膳食中，然后量化膳食模式。总摄入量，由FCM软件评估，在良好的一致性，通过一个独立的量表测量。1千卡的最小膳食量和15分钟的膳食终止标准占总摄入量的90%以上，并产生与文献记录一致的膳食动力学。使用自助餐饮食可以比较单一食物与多食物饮食的膳食模式，以及分析不同膳食子集中与宏量营养素相关的食物选择。FCM被证明可以准确地测量22小时内的食物摄入量，并能够检测出大鼠膳食模式的差异和相似性，作为性别和食物选择可用性的函数。结合任何数量的实验操作，FCM在研究饮食环境中摄食行为的生理和神经控制方面有很大的希望，饮食环境允许食物选择，更紧密地模仿人类的饮食条件。
The measurement of the size and timing of meals provides critical insight into the processes underlying food intake. While most work has been conducted with a single food or fluid, the availability of food choices can also influence eating and interact with these processes. The 5-Item Food Choice Monitor (FCM), a device that continuously measures eating and drinking behaviors of rats provided up to 5 foods and 2 fluids simultaneously, was designed to allow study of food choices simultaneously with meal patterns. To validate this device, adult male and female (n=8 each) Sprague-Dawley rats were housed in the FCM. Food and fluid intake were measured continuously (22-h/day) while rats were presented water and powdered chow. Then a cafeteria diet of 5 foods varying in macronutrient content, texture, and flavors were offered along with water. Lastly, the 5 foods were offered along with 0.3 M sucrose and water. Analyses were conducted to find optimal criteria for parceling ingestive behavior into meals, then meal patterns were quantified. Total intake, as assessed by FCM software, was in good concordance with that measured by an independent scale. A minimum meal size of 1 kcal and a meal termination criterion of 15-min accounted for >90% of total intake and produced meal dynamics that were in register with the literature. Use of the cafeteria diet allowed comparisons between meal patterns with a single food versus a multi-food diet, as well as analyses of macronutrient-related food choices across subsets of meals. The FCM proved to accurately measure food intake over a 22-h period and was able to detect differences and similarities in the meal patterns of rats as a function of sex and food choice availability. Combined with any number of experimental manipulations, the FCM holds great promise in the investigation of the physiological and neural controls of ingestive behavior in a dietary environment that allows food choices, more closely emulating human eating conditions.
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影响因子：
5.9
作者：
Blonde GD;Price RK;le Roux CW;Spector AC
通讯作者：
Spector AC
影响因子：
4.2
作者：
de Castro, JM
通讯作者：
de Castro, JM
影响因子：
2.9
作者：
ALEXANDER, JT;CHEUNG, WK;LEIBOWITZ, SF
通讯作者：
LEIBOWITZ, SF
DOI：
10.1037/h0045421
发表时间：
1960-01-01
期刊：
JOURNAL OF COMPARATIVE AND PHYSIOLOGICAL PSYCHOLOGY
影响因子：
--
作者：
BOLLES, RC
通讯作者：
BOLLES, RC
影响因子：
--
作者：
BRESLIN, PAS;KAPLAN, JM;GRILL, HJ
通讯作者：
GRILL, HJ