Differentiation of monocytes into multinucleated giant bone-resorbing cells: two-step differentiation induced by nurse-like cells and cytokines.
Differentiation of monocytes into multinucleated giant bone-resorbing cells: two-step differentiation induced by nurse-like cells and cytokines.
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DOI：
10.1186/ar320
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Ochi T
中科院分区：
文献类型：
--
作者：
Toyosaki-Maeda T;Takano H;Tomita T;Tsuruta Y;Maeda-Tanimura M;Shimaoka Y;Takahashi T;Itoh T;Suzuki R;Ochi T
关节骨吸收是类风湿性关节炎(RA)患者的特征性表现。破骨细胞样细胞存在于滑膜组织中，侵袭类风湿关节炎患者的骨。这些细胞的特征还不完全清楚。在这里报告的工作中，我们从健康人的外周血单核细胞中产生了这些细胞。单核细胞与类风湿关节炎患者滑膜组织中的护士样细胞共培养(RA-NLCS)。在培养的5周内，单核细胞被激活并分化为CD14和抗酒石酸酸性磷酸酶(TRAP)阳性的单核细胞。在IL-3、IL-5、IL-7和/或粒细胞-巨噬细胞集落刺激因子的刺激下，这些单个核细胞分化为多核巨骨吸收细胞。类风湿关节炎患者滑液中也有类似特征的TRAP阳性细胞。这些结果表明，多核巨骨吸收细胞由单核细胞分化为两个步骤：首先，RA-NLCS诱导单核细胞分化为TRAP阳性的单核细胞，然后在细胞因子的诱导下分化为多核巨骨吸收细胞。
Bone resorption in the joints is the characteristic finding in patients with rheumatoid arthritis (RA). Osteoclast-like cells are present in the synovial tissues and invade the bone of patients with RA. The characteristics of these cells are not completely known. In the work reported here, we generated these cells from peripheral-blood monocytes from healthy individuals. The monocytes were co-cultured with nurse-like cells from synovial tissues of patients with RA (RA-NLCs). Within 5 weeks of culture, the monocytes were activated and differentiated into mononuclear cells positive for CD14 and tartrate-resistant acid phosphatase (TRAP). These mononuclear cells then differentiated into multinucleated giant bone-resorbing cells after stimulation with IL-3, IL-5, IL-7, and/or granulocyte-macrophage-colony-stimulating factor. TRAP-positive cells with similar characteristics were found in synovial fluid from patients with RA. These results indicate that multinucleated giant bone-resorbing cells are generated from monocytes in two steps: first, RA-NLCs induce monocytes to differentiate into TRAP-positive mononuclear cells, which are then induced by cytokines to differentiate into multinucleated giant bone-resorbing cells.
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DOI：
10.1073/pnas.93.6.2588
发表时间：
1996-03-19
影响因子：
11.1
作者：
Zhou, LJ;Tedder, TF
通讯作者：
Tedder, TF
DOI：
10.1073/pnas.89.19.9000
发表时间：
1992-10-01
影响因子：
11.1
作者：
IWABUCHI, K;NAKAYAMA, K;LOH, DY
通讯作者：
LOH, DY
影响因子：
6.2
作者：
Higuchi, S;Tabata, N;Ito, Y
通讯作者：
Ito, Y
影响因子：
15.9
作者：
Shimaoka, Y;Attrep, JF;Lipsky, PE
通讯作者：
Lipsky, PE
影响因子：
15.9
作者：
Burger, JA;Zvaifler, NJ;Kipps, TJ
通讯作者：
Kipps, TJ