Glutamine in suppression of lipopolysaccharide-induced piglet intestinal inflammation: The crosstalk between AMPK activation and mitochondrial function.
Glutamine in suppression of lipopolysaccharide-induced piglet intestinal inflammation: The crosstalk between AMPK activation and mitochondrial function.
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谷氨酰胺抑制脂多糖诱导的仔猪肠道炎症：AMPK 激活与线粒体功能之间的串扰
DOI：
10.1016/j.aninu.2022.03.001
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发表时间：
2022-09
期刊：
影响因子：
6.3
通讯作者：
Yin, Yulong
中科院分区：
文献类型：
--
作者：
He, Liuqin;Zhou, Xihong;Wu, Ziping;Feng, Yanzhong;Liu, Di;Li, Tiejun;Yin, Yulong
本研究旨在探讨谷氨酰胺（Gln）对大肠杆菌脂多糖（E. coli LPS）诱导的肠道炎症的调节机制。选用21日龄断奶仔猪（n = 8），分别饲喂基础日粮（对照组和LPS组）和1%Gln日粮（Gln + LPS组），试验期4周。在第22、24、26和28天，LPS组和Gln + LPS组腹腔注射大肠杆菌LPS。采用LPS诱导的猪肠上皮细胞（IPEC-J2）（n = 6）研究相关机制，并采用化合物C抑制腺苷5′-磷酸活化蛋白激酶（AMPK）活性。结果表明，与LPS处理相比，Gln + LPS处理具有更好的生长性能和更高的绒毛高度（P < 0.05），Gln + LPS治疗使腹泻率降低6.4%（P < 0.05）; Gln + LPS处理降低血清肿瘤坏死因子（TNF-α）、白细胞介素-6（IL-6）、K+、皮质醇和胰岛素水平，Gln + LPS处理组空肠和回肠水通道蛋白和AMPK通路相关靶蛋白的表达显著增加（P < 0.05），离子转运蛋白的表达显著降低（P < 0.05）。体外实验结果表明，4 mmol/L Gln可抑制LPS诱导的IPEC-J2细胞凋亡（P < 0.05），抑制细胞分泌白细胞介素1β（IL-1β）、IL-6和TNF-α，促进线粒体呼吸代谢（P <0.05），增加线粒体数量和线粒体膜电位（P < 0.05）。在LPS刺激或正常条件下，Gln处理的IPEC-J2细胞中AMPK活性升高70%~ 300%。我们的研究结果表明，预先给予谷氨酰胺仔猪通过调节AMPK激活和线粒体功能之间的串扰抑制肠道炎症。
The study was conducted to investigate the regulatory mechanism of glutamine (Gln) on intestinal inflammation in an Escherichia coli lipopolysaccharide (E. coli LPS)-induced in vivo and in vitro models. Piglets (n = 8) weaned at 21 d of age were fed a basal diet (control and LPS groups) or 1% Gln diet (Gln + LPS group) ad libitum for 4 weeks. On d 22, 24, 26 and 28, piglets in the LPS and Gln + LPS groups were intraperitoneally injected with E. coli LPS. Intestinal porcine epithelial cells (IPEC-J2) (n = 6) induced by LPS were used to assess related mechanisms and compound C was used to inhibit adenosine 5′-monophosphate-activated protein kinase (AMPK) activity. Our current results showed that compared with the LPS treatment, the Gln + LPS treatment had better growth performance and greater villus height (P < 0.05), and the Gln + LPS treatment reduced the rate of diarrhea by 6.4% (P < 0.05); the Gln + LPS treatment decreased serum tumor necrosis factor (TNF-ɑ), interleukin-6 (IL-6), K+, cortisol and insulin levels, whereas increased (P < 0.05) serum immunoglobulin M and epidermal growth factor levels; the Gln + LPS treatment increased (P < 0.05) the expression of aquaporins and AMPK pathway-associated targets in the jejunum and ileum of piglets, whereas decreased the expression of ion transporters (P < 0.05). The in vitro results showed that 4 mmol/L Gln administration could inhibit (P < 0.05) cell apoptosis and interleukin-1β (IL-1β), IL-6 and TNF-ɑ secretion in LPS-induced IPEC-J2 cells, promote (P < 0.05) mitochondrial respiratory metabolism and increase (P < 0.05) the number of mitochondria and mitochondrial membrane potential. The activity of AMPK was elevated by 70% to 300% in Gln-treated IPEC-J2 cells under LPS challenge or normal conditions. Our results indicate that pre-administration of Gln to piglets suppresses intestinal inflammation by modulating the crosstalk between AMPK activation and mitochondrial function.
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影响因子：
2.5
作者：
Peplowski MA;Vegso AJ;Iablokov V;Dicay M;Zaheer RS;Renaux B;Proud D;Hollenberg MD;Beck PL;MacNaughton WK
通讯作者：
MacNaughton WK
影响因子：
8
作者：
Ho GT;Aird RE;Liu B;Boyapati RK;Kennedy NA;Dorward DA;Noble CL;Shimizu T;Carter RN;Chew ETS;Morton NM;Rossi AG;Sartor RB;Iredale JP;Satsangi J
通讯作者：
Satsangi J
影响因子：
5.6
作者：
Kłos P;Dabravolski SA
通讯作者：
Dabravolski SA
影响因子：
64.8
作者：
Piskounova E;Agathocleous M;Murphy MM;Hu Z;Huddlestun SE;Zhao Z;Leitch AM;Johnson TM;DeBerardinis RJ;Morrison SJ
通讯作者：
Morrison SJ
影响因子：
3.2
作者：
Robinson K;Deng Z;Hou Y;Zhang G
通讯作者：
Zhang G