Localization of high level of sequence conservation and divergence regions in cotton
Localization of high level of sequence conservation and divergence regions in cotton
复制标题
棉花高水平序列保守和分歧区域的定位
DOI：
10.1007/s00122-011-1777-3
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发表时间：
2012-01
影响因子：
5.4
通讯作者：
Zhang T.
中科院分区：
文献类型：
--
作者：
Wang, Kai;Zhang, W;Cao, Y;Zhang, Z;Zheng, D;Zhou, B;Guo, W;Zhang T.
在先前的研究中，我们观察到的染色体大小的变化是由于不均匀的扩展和收缩，通过比较的结构和大小的一对同源高分辨率的细胞遗传学图的12 A和12 D在四倍体棉花。为了揭示序列水平上的变异，在本文中，我们对两对同源细菌人工染色体进行了测序，这些人工染色体来源于基因组的高变区和低变区。比较它们的序列变异，证实了高度保守和差异的序列存在于远端和臂间区，高和低可变基因组大小区域在这两对棉花同源染色体。序列分析也证实了Gossypiumretrotransposablegypsy-like元件（Gorge 3）的差异积累是造成臂间区大小差异的主要原因。通过荧光原位杂交分析，发现Gorge 3在AT/A近端区域呈偏性分布，并与整个基因组中的异染色质沿着分布。这些结果表明，在棉花AT/A和DT/D基因组之间，末端和臂间区通常分别具有高度的序列保守性和分歧。
In a previous study, we observed that the variations in chromosome size are due to uneven expansion and contraction by comparing the structures and sizes of a pair of homoeologous high-resolution cytogenetic maps of chromosomes 12A and 12D in tetraploid cotton. To reveal the variation at the sequence level, in the present paper, we sequenced two pairs of homoeologous bacterial artificial chromosomes derived from high- to low-variable genomic regions. Comparisons of their sequence variations confirmed that the highly conserved and divergent sequences existed in the distal and pericentric regions, e.g., high- and low-variable genome size regions in these two pairs of cotton homoeologous chromosomes. Sequence analysis also confirmed that the differential accumulation ofGossypiumretrotransposablegypsy-like element (Gorge3) accounted for the main contributions for the size difference between the pericentric regions. By fluorescence in situ hybridization analysis, we found thatGorge3has a bias distribution in the AT/A proximal regions and is associated with the heterochromatin along the chromosomes in the entireGossypiumgenome. These results indicate that, between AT/A and DT/D genomes, the distal and pericentric regions usually possess high level of sequence conservation and divergence, respectively, in cotton.
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影响因子：
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作者：
Jurka, J;Kapitonov, VV;Walichiewicz, J
通讯作者：
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影响因子：
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DOI：
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发表时间：
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影响因子：
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