Identification of circRNAs and circRNA-miRNA-mRNA regulatory network in radiation-induced heart disease
Identification of circRNAs and circRNA-miRNA-mRNA regulatory network in radiation-induced heart disease
复制标题
辐射诱发心脏病中 circRNA 和 circRNA-miRNA-mRNA 调控网络的鉴定
DOI：
10.1080/09553002.2023.2176560
复制
发表时间：
2023-02
影响因子：
2.6
通讯作者：
Li Shen
中科院分区：
文献类型：
--
作者：
Shutong Shen;Ping Lyu;Baixia Yang;Xi Yang;Yida Li;Zhengfei Zhu;Li Shen
摘要目的放射性心脏病（RIHD）是胸部放疗后最常见、最严重的远期不良反应之一。我们的目的是研究潜在的分子机制RIHD使用RNA测序（RNA-seq）和生物信息学方法。材料与方法建立RIHD大鼠模型，并使用RNA-seq鉴定转录谱。鉴定了差异表达的circRNA、miRNA和mRNA。基于GO和KEGG数据库进行功能富集和信号通路分析。建立了RIHD潜在的circRNA-miRNA-mRNA调控网络。qRT-PCR用于验证相关基因。结果RIHD中有21个circRNA、26个miRNAs和178个mRNA转录本差异表达。GO和KEGG通路分析表明，差异表达的mRNA在涉及内皮功能和血管病理过程的通路中最丰富。其中9种circRNA、10种miRNA和6种mRNA转录本最可能参与血管功能，并建立了circRNA-miRNA-mRNA的候选竞争性内源性RNA（ceRNA）网络，qRT-PCR进一步验证了该网络的有效性。结论血管病变在RIHD早期起重要作用。此外，还发现了一个circRNA-miRNA-mRNA ceRNA网络，该网络可能参与了血管功能和RIHD的调节。
Abstract Objective Radiation-induced heart disease (RIHD) is one of the most common and serious long-term adverse effect after thoracic radiotherapy. Our aim was to investigate the potential molecular mechanism underlying RIHD using RNA-sequencing (RNA-seq) and bioinformatics methods. Materials and methods An RIHD rat model was established and transcription profiles were identified using RNA-seq. Differentially expressed circRNAs, miRNAs and mRNAs were identified. Enrichment of functions and signaling pathways analysis were performed based on GO and the KEGG database. Potential circRNA-miRNA-mRNA regulatory network underlying RIHD was established. qRT-PCR was used to validate the associated genes. Results In total, 21 circRNAs, 26 miRNAs, and 178 mRNA transcripts were differentially expressed in RIHD. GO and KEGG pathway analyses identified that differentially expressed mRNAs were most enriched in pathways referring to endothelial function and vascular pathological processes. Nine circRNAs, 10 miRNAs, and 6 mRNA transcripts were most likely involved in vascular function and a candidate competitive endogenous RNA (ceRNA) network of circRNA-miRNA-mRNA was established, which were further validated by qRT-PCR. Conclusions Our study revealed that vascular pathology plays an important role in the early stage of RIHD. Furthermore, a circRNA-miRNA-mRNA ceRNA network was found that may be involved in the regulation of vascular function and RIHD.
登录
查看更多内容
钙网蛋白通过促进肌动蛋白乙酰化和聚合减轻微波辐射引起的人微血管内皮细胞损伤
DOI：
10.1007/s12192-016-0745-x
发表时间：
2016
期刊：
Cell Stress Chaperones
影响因子：
--
作者：
Xu FF;Wang Y;Tao TQ;Song DD;Liu XH
通讯作者：
Liu XH
DOI：
10.1038/s41569-020-0352-5
发表时间：
2020-07
期刊：
Nature reviews. Cardiology
影响因子：
--
作者：
Saigusa R;Winkels H;Ley K
通讯作者：
Ley K
影响因子：
1.5
作者：
Viczenczova, Csilla;Bacova, Barbara Szeiffova;Tribulova, Narcis
通讯作者：
Tribulova, Narcis
DOI：
10.1016/j.bbrc.2011.09.150
发表时间：
2011-11
影响因子：
3.1
作者：
M. Rousseau;M. Gaugler;A. Rodallec;S. Bonnaud;F. Paris;I. Corre
通讯作者：
M. Rousseau;M. Gaugler;A. Rodallec;S. Bonnaud;F. Paris;I. Corre
DOI：
10.26355/eurrev_202005_21192
发表时间：
2020-05-01
影响因子：
3.3
作者：
Li, L-L;Mao, C-D;Xue, A-G
通讯作者：
Xue, A-G