A modified consumer inkjet for spatiotemporal control of gene expression.
A modified consumer inkjet for spatiotemporal control of gene expression.
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DOI：
10.1371/journal.pone.0007086
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发表时间：
2009-09-18
期刊：
影响因子：
3.7
通讯作者：
Maharbiz MM
中科院分区：
文献类型：
--
作者：
Cohen DJ;Morfino RC;Maharbiz MM
本文提出了一种低成本的喷墨给药系统，能够连续的，二维时空的基因表达调控，通过交付可扩散的调节剂，以定制安装的凝胶培养的大肠杆菌。杆菌消费级喷墨打印机适用于化学打印;大肠杆菌培养物在750 μm厚的琼脂上生长，琼脂包埋在机加工成商业光盘的微孔中。乳糖操纵子的时空调节通过将乳糖和葡萄糖的图案直接印刷到培养物中来证明; X-Gal蓝色图案用于视觉反馈。我们展示了乳糖操纵子的反馈的非线性，当乳糖（诱导剂）和葡萄糖（抑制剂）的图案扰动，可以导致协调的方式，模仿图案在发育生物学领域的细胞表达模式。这方面的例子包括尖锐的边界和mRNA表达行波的产生。据我们所知，这是第一次证明反应扩散效应在充分研究乳糖操纵子。一个有限元反应扩散模型的紫胶操纵子预测模式的形成具有良好的保真度。
This paper presents a low-cost inkjet dosing system capable of continuous, two-dimensional spatiotemporal regulation of gene expression via delivery of diffusible regulators to a custom-mounted gel culture of E. coli. A consumer-grade, inkjet printer was adapted for chemical printing; E. coli cultures were grown on 750 µm thick agar embedded in micro-wells machined into commercial compact discs. Spatio-temporal regulation of the lac operon was demonstrated via the printing of patterns of lactose and glucose directly into the cultures; X-Gal blue patterns were used for visual feedback. We demonstrate how the bistable nature of the lac operon's feedback, when perturbed by patterning lactose (inducer) and glucose (inhibitor), can lead to coordination of cell expression patterns across a field in ways that mimic motifs seen in developmental biology. Examples of this include sharp boundaries and the generation of traveling waves of mRNA expression. To our knowledge, this is the first demonstration of reaction-diffusion effects in the well-studied lac operon. A finite element reaction-diffusion model of the lac operon is also presented which predicts pattern formation with good fidelity.
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影响因子：
56.9
作者：
Amonlirdviman, K;Khare, NA;Tomlin, CJ
通讯作者：
Tomlin, CJ
影响因子：
--
作者：
Cohen, Daniel L.;Malone, Evan;Bonassar, Lawrence J.
通讯作者：
Bonassar, Lawrence J.
影响因子：
4.7
作者：
Boland, Thomas;Xu, Tao;Cui, Xiaofeng
通讯作者：
Cui, Xiaofeng
影响因子：
2.5
作者：
Chambers, James J.;Banghart, Matthew R.;Kramer, Richard H.
通讯作者：
Kramer, Richard H.
DOI：
10.1073/pnas.0404353101
发表时间：
2004-11-09
影响因子：
11.1
作者：
Gu, W;Zhu, XY;Takayama, S
通讯作者：
Takayama, S