Assessment of fishery resources using environmental DNA: Small yellow croaker (Larimichthys polyactis) in East China Sea.
Assessment of fishery resources using environmental DNA: Small yellow croaker (Larimichthys polyactis) in East China Sea.
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利用环境DNA评估渔业资源：东海小黄鱼（Larimichthys polyactis）
DOI：
10.1371/journal.pone.0244495
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Gao T
中科院分区：
文献类型：
--
作者：
Wang X;Lu G;Zhao L;Yang Q;Gao T
物种分布监测和生物量评估对渔业管理和资源保护至关重要。然而，与新的环境DNA(EDNA)方法相比，传统的方法，如机动拖网，成本较高，效果较差。采用EDNA方法对东中国海区重要经济鱼种小黄鱼的水平和垂直分布进行了调查。利用物种特异性引物和Taqman探针对44个采样点采集的171个埃德纳样品进行分析，结果表明44个采样点的28个采样层中存在小黄鱼。小黄鱼EDNA浓度在不同采样点和不同层级之间存在显著差异，这与前人通过近海和近岸鱼类浮游动物调查所得的结果一致，表明小黄鱼表现出较强的区域分布和层间偏好。此外，我们在机动拖网禁渔线以外的表层水域发现了高度EDNA密度的小黄鱼，这证实了产卵场已从近岸扩大到近海地区。这种产卵场的扩大可能是小黄鱼对过度捕捞、气候变化和近岸环境污染等压力因素的反应。为了识别可能与小黄鱼有无相关的环境变量，我们进行了EDNA浓度与环境变量之间的相关分析，结果为未来渔业资源的进一步调查提供了指导。总之，这项研究证明了EDNA方法在大范围地理范围内监测小黄鱼方面的力量。预计制定的方案和结果将有助于长期监测和保护计划，并有利于小黄鱼的可持续渔业。
Species distribution monitoring and biomass assessment are crucial for fishery management and resource conservation. However, traditional methods such as motor trawling are costly and less effective than the novel environmental DNA (eDNA) approach. This study employs eDNA approach to investigate horizontal and vertical distributions of small yellow croaker (Larimichthys polyactis), an economically important species, in the East China Sea. The analysis of 171 eDNA samples collected from 44 stations using the species-specific primers and Taqman probe suggests a presence of small yellow croaker at 28 sampling layers in 44 stations. Significant differences in croaker eDNA concentrations were revealed among sampling stations and layers, consistent with previous findings through motor-trawl capture offshore and nearshore ichthyoplakton surveys, indicating small yellow croaker exhibits strong regional distribution and layer preference. In addition, we found a high eDNA concentration of small yellow croaker in the surface waters beyond the motor-trawl prohibition line, which confirms spawning grounds have been expanded from nearshore to offshore areas. Such expansion of spawning grounds could be a response by small yellow croaker to stressors such as overfishing, climate change, and nearshore environment contamination. To identify environmental variables potentially associated with small yellow croaker presence and absence, we conducted a correlation analysis between eDNA concentration and environmental variables, and the results provide a guideline for further investigation of fishery resources in the future. In conclusion, this study demonstrates the power of the eDNA approach in monitoring small yellow croaker at extensive geographic scales. The developed protocols and the findings are expected to assist in long-term monitoring and protection programs and benefit sustainable fishery in small yellow croaker.
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影响因子：
3.5
作者：
Miya M;Sato Y;Fukunaga T;Sado T;Poulsen JY;Sato K;Minamoto T;Yamamoto S;Yamanaka H;Araki H;Kondoh M;Iwasaki W
通讯作者：
Iwasaki W
影响因子：
1.4
作者：
Lin Longshan;Chen Yongjun;Shao Kwang-tsao
通讯作者：
Shao Kwang-tsao
影响因子：
3.7
作者：
Thomsen PF;Kielgast J;Iversen LL;Møller PR;Rasmussen M;Willerslev E
通讯作者：
Willerslev E
影响因子：
2.5
作者：
Cheung, William W. L.;Close, Chris;Pauly, Daniel
通讯作者：
Pauly, Daniel
影响因子：
--
作者：
Lin Longshan;Liu Zunlei;Gao Tianxiang
通讯作者：
Gao Tianxiang