Expression of cystic fibrosis transmembrane conductance regulator in ganglion cells of the hearts
Expression of cystic fibrosis transmembrane conductance regulator in ganglion cells of the hearts
复制标题
囊性纤维化跨膜电导调节因子在心脏神经节细胞中的表达
DOI：
10.1016/j.neulet.2008.05.087
复制
发表时间：
2008
影响因子：
2.5
通讯作者：
J. Gu
中科院分区：
文献类型：
--
作者：
Peng Pan;Yong Guo;J. Gu
囊性纤维化跨膜传导调节因子（CFTR）作为一种重要的氯离子选择性通道，分布于分泌氯离子的上皮细胞的顶膜。然而，CFTR也被报道在人类和大鼠脑的神经元中表达。在这项研究中，我们的目的是调查CFTR在神经节细胞的心脏。我们采用免疫组织化学、原位杂交、激光显微切割（LMD）和巢式逆转录聚合酶链反应（nested RT-PCR）等方法检测了大鼠心脏神经节细胞CFTR的表达，发现CFTR蛋白及其mRNA在大鼠心脏神经节细胞中广泛而丰富地表达。CFTR在神经节中的存在不仅为囊性纤维化患者的心血管症状提供了可能的解释，而且还可能导致更好地理解CFTR在心脏神经元调节中的可能作用。
Cystic fibrosis transmembrane conductance regulator (CFTR) as an important chloride-selective channel is known to distribute on the apical membrane of chloride-secreting epithelial cells. However, CFTR is also reported to express in the neurons of human and rat brain. In this study we aim to investigate the expression of CFTR in ganglion cells of the hearts. We used immunohistochemistry, in situ hybridization, laser microdissection (LMD) and nested reverse transcriptase polymerase chain reaction (nested RT-PCR) to detect CFTR in the ganglion cells of the Sprague–Dawley rat hearts and found widespread and abundant the expression of CFTR protein and its mRNA in the ganglion cells of the rat hearts. The presence of CFTR in ganglia does not only provide a possible explanation for cardiovascular symptoms of cystic fibrosis patients but also may lead to a better understanding of a possible role for CFTR in the neuronal regulation of the heart.
影响因子：
15.9
作者：
MULBERG, AE;RESTA, LP;BROUSSARD, DL
通讯作者：
BROUSSARD, DL
影响因子：
1.7
作者：
A. Mulberg;E. Wiedner;X. Bao;J. Marshall;D. Jefferson;S. Altschuler
通讯作者：
A. Mulberg;E. Wiedner;X. Bao;J. Marshall;D. Jefferson;S. Altschuler
CFTR 亚细胞定位于导管上皮内体。
DOI：
10.1152/ajpcell.1994.267.2.c340
发表时间：
1994
期刊：
The American journal of physiology
影响因子：
--
作者：
Webster,P;Vanacore,L;Nairn,AC;Marino,CR
通讯作者：
Marino,CR