Validation of a new larval rearing unit for Aedes albopictus (Diptera: Culicidae) mass rearing.
Validation of a new larval rearing unit for Aedes albopictus (Diptera: Culicidae) mass rearing.
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DOI：
10.1371/journal.pone.0091914
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Bellini R
中科院分区：
文献类型：
--
作者：
Balestrino F;Puggioli A;Gilles JR;Bellini R
对粮农组织/原子能机构联合司昆虫害虫控制实验室开发的蚊子幼虫饲养装置进行了评价，以确定其用于白纹伊蚊（Skuse，1895年）大规模饲养的可能性，从而支持为该物种开发昆虫不育技术（SIT）包。大量饲养托盘和机架的使用没有不利影响幼虫的发育，化蛹和存活率，并允许与传统的单个托盘相比，减少空间需求的大型幼虫饲养菌落的管理。幼虫密度，水温和饮食组成对蛹生产和性别分离效力的大小分化的影响进行了分析，为个人的大量饲养托盘，以及在专用机架单元内堆叠的多个托盘。在28°C的温度下，在由50%金枪鱼粉、36%牛肝粉、14%啤酒酵母和作为添加剂的每100 ml饮食溶液0.2克维生素混合物组成的饮食上，以每ml去离子水3只幼虫的密度，使用每托盘18000只幼虫获得最佳结果。在L1期幼虫引入后第6天收获蛹，并使用1400 µm筛分离雄性，准确率为99.0%，回收率约为100%。占所有可用男性的25%。使用这种幼虫饲养装置，在2平方米的实验室空间内，每周平均可生产100，000只雄性蛹。与以往的实验室饲养方法相比，只有使用钙才能达到相同的蛹产量和性别分离效果。200个塑料托盘，需要两个5平方米的气候控制室。
The mosquito larval rearing unit developed at the Insect Pest Control Laboratory (IPCL) of the FAO/IAEA Joint Division was evaluated for its potential use for Aedes albopictus (Skuse, 1895) mass rearing in support of the development of a sterile insect technique (SIT) package for this species. The use of the mass rearing trays and rack did not adversely affect larval development, pupation and survival rates and allowed the management of large larval rearing colonies with reduced space requirements in comparison with classical individual trays. The effects of larval density, water temperature and diet composition on pupal production and size differentiation for sex separation efficacy were analyzed for individual mass rearing trays as well as multiple trays stacked within the dedicated rack unit. Best results were obtained using eighteen thousand larvae per tray at a density of 3 larvae per ml of deionized water at a temperature of 28°C on a diet consisting of 50% tuna meal, 36% bovine liver powder, 14% brewer's yeast and, as an additive, 0.2 gr of Vitamin Mix per 100 ml of diet solution. Pupae were harvested on the sixth day from larval introduction at L1 stage and males were separated out by the use of a 1400 µm sieve with 99.0% accuracy with a recovery rate of ca. 25% of the total available males. With the use of this larval rearing unit, an average production of 100,000 male pupae per week can be achieved in just 2 square meter of laboratory space. Compared to previous laboratory rearing method, the same pupal production and sex separation efficacy could only be achieved by use of ca. 200 plastic trays which required the space of two 5 square meter climatic-controlled rooms.
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影响因子：
2.2
作者：
Barrera, R
通讯作者：
Barrera, R
DOI：
10.1017/s0007485300053499
发表时间：
1958-01-01
期刊：
BULL ENT RES
影响因子：
--
作者：
BAR-ZEEV, MICHA
通讯作者：
BAR-ZEEV, MICHA
影响因子：
1
作者：
Balestrino, Fabrizio;Gilles, Jeremie R. L.;Benedict, Mark Q.
通讯作者：
Benedict, Mark Q.
影响因子：
11.8
作者：
Gubler DJ
通讯作者：
Gubler DJ
影响因子：
2.1
作者：
Damiens, D.;Benedict, M. Q.;Gilles, J. R. L.
通讯作者：
Gilles, J. R. L.