Can shellfish be used to monitor SARS-CoV-2 in the coastal environment?
Can shellfish be used to monitor SARS-CoV-2 in the coastal environment?
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DOI：
10.1016/j.scitotenv.2021.146270
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发表时间：
2021-07-15
期刊：
影响因子：
--
通讯作者：
Le Guyader FS
中科院分区：
文献类型：
--
作者：
Desdouits M;Piquet JC;Wacrenier C;Le Mennec C;Parnaudeau S;Jousse S;Rocq S;Bigault L;Contrant M;Garry P;Chavanon F;Gabellec R;Lamort L;Lebrun L;Le Gall P;Meteigner C;Schmitt A;Seugnet JL;Serais O;Peltier C;Bressolette-Bodin C;Blanchard Y;Le Guyader FS
SARS-CoV-2的出现和全球传播引起了新的关注和挑战，即这种病毒可能通过人类污水的溢出而污染环境，在那里已经发现了这种病毒。在日益增加的人为压力下，沿海环境受到来自污水的大量人类病毒的污染，其中大多数是无包膜病毒，如诺如病毒。当它们到达沿海沃茨时，可通过滤食性贝类（如牡蛎）进行生物积累。建立了检测这种病毒污染的方法，用于检测无包膜肠道病毒，可能需要优化以适应包膜病毒，如冠状病毒（CoV）。在这里，我们的目的是评估方法的检测冠状病毒，包括SARS-CoV-2，在沿海环境和测试的可能性，SARS-CoV-2可以污染牡蛎，监测污染的法国海岸的SARS-CoV-2使用海水和贝类。使用猪流行性腹泻病毒（PEDV），冠状病毒，作为替代SARS-CoV-2，杜兰病毒，作为替代无包膜病毒，如诺如病毒，我们评估和选择的方法来检测CoV在海水和贝类。基于海水的方法显示出PEDV的可变和低产量。在贝类中，目前的诺如病毒检测规范适用于CoV检测。PEDV和热灭活的SARS-CoV-2都可以在实验室环境中污染牡蛎，其效率低于用作对照的杯状病毒。最后，我们将我们的方法应用于2020年4月至8月在法国收集的海水和贝类样本，我们可以检测到人类诺如病毒（人类粪便污染的标志物）的存在，但不能检测到SARS-CoV-2。总之，我们的研究结果验证了在沿海环境中检测CoV的方法，包括使用贝类作为其环境微生物质量的哨兵，并表明SARS-CoV-2在夏季没有污染法国海岸。
The emergence and worldwide spread of SARS-CoV-2 raises new concerns and challenges regarding possible environmental contamination by this virus through spillover of human sewage, where it has been detected. The coastal environment, under increasing anthropogenic pressure, is subjected to contamination by a large number of human viruses from sewage, most of them being non-enveloped viruses like norovirus. When reaching coastal waters, they can be bio-accumulated by filter-feeding shellfish species such as oysters. Methods to detect this viral contamination were set up for the detection of non-enveloped enteric viruses, and may need optimization to accommodate enveloped viruses like coronaviruses (CoV). Here, we aimed at assessing methods for the detection of CoV, including SARS-CoV-2, in the coastal environment and testing the possibility that SARS-CoV-2 can contaminate oysters, to monitor the contamination of French shores by SARS-CoV-2 using both seawater and shellfish. Using the porcine epidemic diarrhea virus (PEDV), a CoV, as surrogate for SARS-CoV-2, and Tulane virus, as surrogate for non-enveloped viruses such as norovirus, we assessed and selected methods to detect CoV in seawater and shellfish. Seawater-based methods showed variable and low yields for PEDV. In shellfish, the current norm for norovirus detection was applicable to CoV detection. Both PEDV and heat-inactivated SARS-CoV-2 could contaminate oysters in laboratory settings, with a lower efficiency than a calicivirus used as control. Finally, we applied our methods to seawater and shellfish samples collected from April to August 2020 in France, where we could detect the presence of human norovirus, a marker of human fecal contamination, but not SARS-CoV-2. Together, our results validate methods for the detection of CoV in the coastal environment, including the use of shellfish as sentinels of the microbial quality of their environment, and suggest that SARS-CoV-2 did not contaminate the French shores during the summer season.
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DOI：
10.1016/j.ijfoodmicro.2018.06.001
发表时间：
2018-11-20
影响因子：
5.4
作者：
Bosch A;Gkogka E;Le Guyader FS;Loisy-Hamon F;Lee A;van Lieshout L;Marthi B;Myrmel M;Sansom A;Schultz AC;Winkler A;Zuber S;Phister T
通讯作者：
Phister T
影响因子：
28.3
作者：
Gorbalenya, Alexander E.;Baker, Susan C.;Ziebuhr, John
通讯作者：
Ziebuhr, John
影响因子：
4.4
作者：
Flannery, John;Keaveney, Sinead;Dore, William
通讯作者：
Dore, William
影响因子：
9.8
作者：
Guerrero-Latorre, Laura;Ballesteros, Isabel;Rios-Touma, Blanca
通讯作者：
Rios-Touma, Blanca
影响因子：
4.4
作者：
Grodzki, Marco;Ollivier, Joanna;Le Guyader, Francoise S.
通讯作者：
Le Guyader, Francoise S.