Long non-coding RNA HCG11 sponging miR-522-3p inhibits the tumorigenesis of non-small cell lung cancer by upregulating SOCS5.
Long non-coding RNA HCG11 sponging miR-522-3p inhibits the tumorigenesis of non-small cell lung cancer by upregulating SOCS5.
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DOI：
10.1111/1759-7714.13624
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发表时间：
2020-10
期刊：
影响因子：
2.9
通讯作者：
Chu F
中科院分区：
文献类型：
--
作者：
Fan G;Jiao J;Shen F;Ren Q;Wang Q;Chu F
大量研究表明，长链非编码RNA（lncRNA）与包括非小细胞肺癌（NSCLC）在内的多种人类疾病有关。本研究的目的是探讨lncRNA HCG 11在NSCLC发病机制中的潜在作用。RT-qPCR检测HCG 11、miR-522 - 3 p和SOCS 5 mRNA表达。通过CCK-8、transwell和双荧光素酶报告基因试验研究lncRNA HCG 11的调控机制。在NSCLC组织和细胞中发现lncRNA HCG 11下调和miR-522 - 3 p上调，lncRNA HCG 11和miR-522 - 3 p的异常表达与NSCLC患者的不良临床结局相关。LncRNA HCG 11充当miR-522 - 3 p的分子海绵。在功能上，lncRNA HCG 11通过下调miR-522 - 3 p抑制NSCLC中的细胞活力、迁移和侵袭。此外，miR-522 - 3 p直接靶向SOCS 5。lncRNAHCG 11对SOCS 5的表达有正调节作用。此外，HCG 11下调或miR-522 - 3 p过表达消除了SOCS 5对NSCLC细胞活力、迁移和侵袭的抑制作用。LncRNA HCG 11通过作为miR-522 - 3 p的ceRNA上调SOCS 5来抑制NSCLC中的细胞活力、迁移和侵袭。lncRNA HCG 11的下调与NSCLC患者的不良临床结局相关已显示HCG 11抑制NSCLC中的细胞活力和运动性，并且HCG 11作为miR-522 - 3 p的ceRNA起作用以上调SOCS 5。
Numerous studies have shown that long non‐coding RNA (lncRNA) is involved in various human diseases including non‐small cell lung cancer (NSCLC). The aim of this study was to explore the potential role of lncRNA HCG11 in the pathogenesis of NSCLC. The mRNA expression of HCG11, miR‐522‐3p and SOCS5 was detected by RT‐qPCR. The regulatory mechanism of lncRNA HCG11 was investigated by CCK‐8, transwell and dual luciferase reporter assays. Downregulation of lncRNA HCG11 and upregulation of miR‐522‐3p were found in NSCLC tissues and cells, and abnormal expressions of lncRNA HCG11 and miR‐522‐3p were related to adverse clinical outcomes of NSCLC patients. LncRNA HCG11 acted as a molecular sponge for miR‐522‐3p. Functionally, lncRNA HCG11 inhibited cell viability, migration and invasion in NSCLC by downregulating miR‐522‐3p. Further, miR‐522‐3p directly targeted SOCS5. lncRNA HCG11 could positively regulate SOCS5 expression in NSCLC. In addition, HCG11 downregulation or miR‐522‐3p overexpression abolished the inhibitory effect of SOCS5 on cell viability, migration and invasion in NSCLC. LncRNA HCG11 inhibits cell viability, migration and invasion in NSCLC by functioning as a ceRNA of miR‐522‐3p to upregulate SOCS5. Downregulation of lncRNA HCG11 is related to adverse clinical outcomes in NSCLC patients. HCG11 has been shown to inhibit cell viability and motility in NSCLC, and HCG11 functions as a ceRNA of miR‐522‐3p to upregulate SOCS5.
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