Comparative transcriptomic analysis of seed coats with high and low lignin contents reveals lignin and flavonoid biosynthesis in Brassica napus.
Comparative transcriptomic analysis of seed coats with high and low lignin contents reveals lignin and flavonoid biosynthesis in Brassica napus.
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DOI：
10.1186/s12870-021-03030-5
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发表时间：
2021-05-29
影响因子：
5.3
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Ding Y;Yu S;Wang J;Li M;Qu C;Li J;Liu L
甘蓝型油菜(2n= 38，AACC）是世界上最重要的油料作物和动物饲料蛋白质来源之一。木质素是一种大分子芳香族聚合物，是细胞壁的主要成分。但种皮中的木质素降低了菜籽饼的利用率，制约了菜籽饼的发展。因此，降低种皮中的木质素含量至关重要。在此，从RIL群体中选择高木质素（H-木质素）和低木质素（L-木质素）含量的重组近交系（RIL）用于分析。横切面结果表明，H-木质素品系的种皮比L-木质素品系厚，尤其是栅栏层。对开花后35、40和46天的种皮和胚进行RNA测序（RNA-Seq），木质素途径中BnPAL和BnC 4 H基因家族的表达在H-木质素种皮中显著高于L-木质素种皮。Bn 4CL基因家族也表现出这种趋势。此外，在与植物激素合成相关的基因中，BnaC 02 g 01710 D在H-木质素系中上调，BnaA 07 g11700 D和BnaC 09 g 00190 D下调。一些转录因子如BnNAC 080、BnNAC 083、BnMYB 9、BnMYB 9 -1、BnMYB 60和BnMYB 60 -1在H-木质素系中上调，而BnMYB 91在H-木质素系中下调。此外，大多数类黄酮途径的基因，如BnCHS和BnDFR，强烈表达的H-木质素种皮。本研究鉴定了与木质素和类黄酮生物合成相关的激素合成基因、转录因子和miRNAs等关键基因。B的监管模式。提出了油菜种皮木质素。这些结果为B中木质素和类黄酮的生物合成提供了新的见解。油菜。在线版本包含补充材料，可通过10.1186/s12870-021-03030-5获得。
Brassica napus L. (2n = 38, AACC) is one of the most important oil crops and sources of protein for animal feed worldwide. Lignin is a large molecule aromatic polymer and a major cell wall component. However, lignin in the seed coat reduces the availability and restricts the development of rapeseed cake. Therefore, it is critical to reduce the lignin content of the seed coat. Here, high-lignin (H-lignin) and low-lignin (L-lignin) content recombinant inbred lines (RILs) were selected from an RIL population for analysis. The cross-section results indicated that the seed coat of the H-lignin lines was thicker than that of the L-lignin lines, especially the palisade layer. The seed coats and embryos at 35, 40 and 46 days after flowering (DAF) were subjected to RNA sequencing (RNA-Seq), and the expression of the BnPAL and BnC4H gene families in the lignin pathway was significantly higher in the H-lignin seed coat than in the L-lignin seed coat. The Bn4CL gene family also showed this trend. In addition, among the genes related to plant hormone synthesis, BnaC02g01710D was upregulated and BnaA07g11700D and BnaC09g00190D were downregulated in H-lignin lines. Some transcription factors were upregulated, such as BnNAC080, BnNAC083, BnMYB9, BnMYB9-1, BnMYB60 and BnMYB60-1, while BnMYB91 was downregulated in H-lignin lines. Moreover, most genes of the flavonoid pathway, such as BnCHS and BnDFR, were strongly expressed in H-lignin seed coat. In Our study, some key genes such as hormone synthesis genes, transcription factors and miRNAs related to lignin and flavonoid biosynthesis were identified. A regulatory model of B. napus seed coat lignin was proposed. These results provide new insight into lignin and flavonoid biosynthesis in B. napus. The online version contains supplementary material available at 10.1186/s12870-021-03030-5.
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