Co-immunoprecipitation-based identification of putative BAX INHIBITOR-1-interacting proteins involved in cell death regulation and plant-powdery mildew interactions.
Co-immunoprecipitation-based identification of putative BAX INHIBITOR-1-interacting proteins involved in cell death regulation and plant-powdery mildew interactions.
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基于免疫共沉淀鉴定参与细胞死亡调节和植物-白粉病相互作用的推定 BAX INHIBITOR-1 相互作用蛋白
DOI：
10.1111/mpp.12050
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发表时间：
2013
影响因子：
4.9
通讯作者：
Hückelhoven R
中科院分区：
文献类型：
--
作者：
Weis C;Pfeilmeier S;Glawischnig E;Isono E;Pachl F;Hahne H;Kuster;Eichmann R;Hückelhoven R
内质网（ER）驻留BAX抑制剂-1（BI-1）蛋白是已知在动物和植物中保守的少数细胞死亡抑制剂之一。BI-1蛋白在响应各种生物和非生物胁迫因素中的功能已得到充分证实。然而，人们对潜在的机制知之甚少。我们进行了免疫共沉淀（co-IP）实验来鉴定拟南芥BI-1相互作用蛋白，以更好地了解BI-1在植物-病原体相互作用过程中的功能以及作为细胞死亡的抑制因子。液相色谱和串联质谱（LC-MS/MS）鉴定了与绿色荧光蛋白（GFP）标记的BI-1共免疫沉淀的95种蛋白质。进一步研究了5种选定的候选蛋白质，核糖体蛋白II（RPN 2）家族蛋白质、液泡ATP合成酶亚单位A（VHA‐A）、细胞色素P450 83 A1（CYP 83 A1）、H+‐ ATP酶1（AHA 1）和抑制素2（PHB 2）在BI‐1相关过程中的作用。为此，我们分析了一组与适应性白粉病真菌十字花科白粉菌相互作用的假突变体和诱导细胞死亡的处理。两个独立的trpn 2敲低突变体倾向于更好地支持白粉病，而aphb 2突变体对诱导细胞死亡的链格孢菌（Alternaria alternataf.sp.lycopersici，AAL）毒素处理表现出改变的反应。两个cyp 83 a1突变体表现出较强的抗白粉病表型，并增强了对AAL毒素的敏感性。此外，共定位研究和荧光共振能量转移（FRET）实验表明BI-1与CYP 83 A1在ER处直接相互作用。
The endoplasmic reticulum (ER)‐resident BAX INHIBITOR‐1 (BI‐1) protein is one of a few cell death suppressors known to be conserved in animals and plants. The function of BI‐1 proteins in response to various biotic and abiotic stress factors is well established. However, little is known about the underlying mechanisms. We conducted co‐immunoprecipitation (co‐IP) experiments to identifyArabidopsis thalianaBI‐1‐interacting proteins to obtain a potentially better understanding of how BI‐1 functions during plant–pathogen interactions and as a suppressor of cell death. Liquid chromatography and tandem mass spectrometry (LC‐MS/MS) identified 95 proteins co‐immunoprecipitated with green fluorescing protein (GFP)‐tagged BI‐1. Five selected candidate proteins, a RIBOPHORIN II (RPN2) family protein, VACUOLAR ATP SYNTHASE SUBUNIT A (VHA‐A), cytochrome P450 83A1 (CYP83A1), H+‐ATPASE 1 (AHA1) and PROHIBITIN 2 (PHB2), were further investigated with regard to their role in BI‐1‐associated processes. To this end, we analysed a set ofArabidopsismutants in the interaction with the adapted powdery mildew fungusErysiphe cruciferarumand on cell death‐inducing treatments. Two independentrpn2knock‐down mutants tended to better support powdery mildew, and aphb2mutant showed altered responses to cell death‐inducingAlternaria alternataf.sp.lycopersici(AAL) toxin treatment. Two independentcyp83a1mutants showed a strong powdery mildew resistance phenotype and enhanced sensitivity to AAL toxin. Moreover, co‐localization studies and fluorescence resonance energy transfer (FRET) experiments suggested a direct interaction of BI‐1 with CYP83A1 at the ER.
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哺乳动物 Bax 通过 ROS 产生和细胞器破坏启动植物细胞死亡。
DOI：
--
发表时间：
2007
期刊：
影响因子：
--
作者：
川合;ら
通讯作者：
ら
DOI：
10.1046/j.1365-313x.2000.00690.x
发表时间：
2000-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Pedro Sanchez;Marta de Torres Zabala;Murray R Grant
通讯作者：
Pedro Sanchez;Marta de Torres Zabala;Murray R Grant
影响因子：
7.3
作者：
Bilodeau, P;Udvardi, MK;Dennis, ES
通讯作者：
Dennis, ES
影响因子：
7.4
作者：
Marco U. Reitz;Jeff Kweku Bissue;K. Zocher;A. Attard;R. Hückelhoven;K. Becker;J. Imani;R. Eichmann-R.-Eichma
通讯作者：
Marco U. Reitz;Jeff Kweku Bissue;K. Zocher;A. Attard;R. Hückelhoven;K. Becker;J. Imani;R. Eichmann-R.-Eichma
影响因子：
11.6
作者：
Kawai-Yamada, M;Ohori, Y;Uchimiya, H
通讯作者：
Uchimiya, H