Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans.
Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans.
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DOI：
10.1371/journal.pone.0165518
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Kuhara A
中科院分区：
文献类型：
--
作者：
Ujisawa T;Ohta A;Uda-Yagi M;Kuhara A
神经系统的温度感知对动物的生命和繁殖至关重要。温度信号传导的分子生理机制尚未完全阐明。我们在这里展示了秀丽隐杆线虫通过三聚体g蛋白信号传导的多种调节机制。分子遗传学研究表明，低温耐受性是由温度感应神经元ASJ中三种Gα蛋白的加性功能调节的，ASJ也被称为光感应神经元。ASJ中钙浓度随温度变化的光学记录表明，三种Gα蛋白在温度信号传导的不同方面起作用。在一个磷酸二酯酶缺陷突变体中，ASJ中钙浓度随温度变化的变化出乎意料地降低，磷酸二酯酶是众所周知的钙增加的负调节因子。总之，这些数据显示了单个感觉神经元中与光和温度信号有关的分子成分的共性和差异。
Temperature sensation by the nervous system is essential for life and proliferation of animals. The molecular-physiological mechanisms underlying temperature signaling have not been fully elucidated. We show here that diverse regulatory machinery underlies temperature sensation through trimeric G-protein signaling in the nematode Caenorhabditis elegans. Molecular-genetic studies demonstrated that cold tolerance is regulated by additive functions of three Gα proteins in a temperature-sensing neuron, ASJ, which is also known to be a light-sensing neuron. Optical recording of calcium concentration in ASJ upon temperature-changes demonstrated that three Gα proteins act in different aspects of temperature signaling. Calcium concentration changes in ASJ upon temperature change were unexpectedly decreased in a mutant defective in phosphodiesterase, which is well known as a negative regulator of calcium increase. Together, these data demonstrate commonalities and differences in the molecular components concerned with light and temperature signaling in a single sensory neuron.
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