Bidirectional temperature-sensing by a single thermosensory neuron in C. elegans.
Bidirectional temperature-sensing by a single thermosensory neuron in C. elegans.
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DOI：
10.1038/nn.2157
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发表时间：
2008-08
影响因子：
25
通讯作者：
Goodman, Miriam B.
中科院分区：
文献类型：
--
作者：
Ramot, Daniel;MacInnis, Bronwyn L.;Goodman, Miriam B.
人类和其他动物可以感受到0.1°C的温度变化。人们对动物如何达到如此精致的敏感性知之甚少。从C.在体内，我们发现冷却关闭和升温打开离子通道。我们发现，AFD热敏性，这超过了许多数量级的大多数生物过程，是通过非线性信号放大。编码环磷酸鸟苷（cGMP）门控离子通道（tax-4和tax-2）和跨膜鸟苷酸环化酶（gcy-8，gcy-18和gcy-23）亚基的基因突变消除了冷却和加热激活的温度感受器电流，表明cGMP介导的途径将温度变化与离子电流变化联系起来。C.的相似之处线虫对温度的敏感性与脊椎动物对光的敏感性之间的关系以及TAX-4和TAX-2与视杆细胞光传导通道亚基之间的序列相似性，提高了线虫温度敏感性与脊椎动物视觉通过保守进化联系起来的可能性。
Humans and other animals can sense temperature changes as small as 0.1°C. How animals achieve such exquisite sensitivity is poorly understood. By recording from the C. elegans thermosensory neurons AFD in vivo, we found that cooling closes and warming opens ion channels. We found that AFD thermosensitivity, which exceeds that of most biological processes by many orders of magnitude, is achieved by nonlinear signal amplification. Mutations in genes encoding subunits of a cyclic guanosine monophosphate (cGMP)-gated ion channel (tax-4 and tax-2) and transmembrane guanylate cyclases (gcy-8, gcy-18 and gcy-23) eliminated both cooling- and warming-activated thermoreceptor currents, indicating that a cGMP-mediated pathway links variations in temperature to changes in ionic currents. The resemblance of C. elegans thermosensation to vertebrate photosensation and the sequence similarity between TAX-4 and TAX-2 and subunits of the rod phototransduction channel raise the possibility that nematode thermosensation and vertebrate vision are linked by conserved evolution.
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DOI：
10.1073/pnas.0406773101
发表时间：
2004-10-26
影响因子：
11.1
作者：
Brauchi, S;Orio, P;Latorre, R
通讯作者：
Latorre, R
影响因子：
64.5
作者：
BARGMANN, CI;HARTWIEG, E;HORVITZ, HR
通讯作者：
HORVITZ, HR
影响因子：
9.2
作者：
Colosimo, ME;Brown, A;Sengupta, P
通讯作者：
Sengupta, P
影响因子：
3
作者：
Goodman, MB;Lockery, SR
通讯作者：
Lockery, SR
影响因子：
56.9
作者：
Kuhara, Atsushi;Okumura, Masatoshi;Mori, Ikue
通讯作者：
Mori, Ikue