First identification of coexistence of blaNDM-1 and blaCMY-42 among Escherichia coli ST167 clinical isolates.
First identification of coexistence of blaNDM-1 and blaCMY-42 among Escherichia coli ST167 clinical isolates.
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DOI：
10.1186/1471-2180-13-282
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发表时间：
2013-12-05
期刊：
影响因子：
4.2
通讯作者：
Yu F
中科院分区：
文献类型：
--
作者：
Zhang X;Lou D;Xu Y;Shang Y;Li D;Huang X;Li Y;Hu L;Wang L;Yu F
肠杆菌科细菌多重耐药性的出现限制了感染性疾病治疗中抗菌药物的选择。NDM-1的鉴定使得治疗多重耐药肠杆菌科感染更加困难。对温州市某三级医院临床分离的耐碳青霉烯类大肠埃希菌进行NDM-1检测。改良Hodge试验阴性，双纸片协同试验阳性。E.大肠杆菌WZ 33和WZ 51对临床常用抗菌药物耐药程度不一致，但对甲氧苄啶/磺胺甲恶唑、阿米卡星、磷霉素、替加环素和多粘菌素B仍敏感。E.经PCR和DNA测序鉴定，大肠杆菌WZ 33和WZ 51均为blaNDM-1阳性。除blaNDM-1外，E. coliWZ 33也含有blaCTX-M-14和blaCMY-42; coliWZ 51同时携带blaSHV-12、blaCTX-M-14和blaCMY-42。对碳青霉烯类抗生素的耐药性。coli WZ 51和WZ 33不能转化E. coli受体，但可转移到E. coli受体进行化学转化。用EcoR 1酶切法对大肠杆菌的质粒DNA进行了分析。coli WZ 51与E.大肠杆菌WZ 51。MLST结果表明，E. coli WZ 33和WZ 51属于动物相关克隆（ST 167）。本研究首次报道了blaNDM-1在大肠杆菌中的携带。coliST 167分离株及blaNDM-1和blaCMY-42在同一分离株中共存。系统监测应重点关注blaNDM-1在肠杆菌科尤其是大肠杆菌中的传播。coliST 167克隆与动物感染相关。
Emergence of multidrug resistance in Enterobacteriaceae limits the selection of antimicrobials for treatment of infectious diseases. Identification of NDM-1 makes more difficulty in treating multidrug-resistant Enterobacteriaceae infections. Carbapenem-resistant Escherichia coli clinical isolates from a tertiary hospital in Wenzhou, east China, were investigated for NDM-1 production. The two tested isolates were negative for modified Hodge test, but positive for a double-disc synergy test used for detecting metallo-β-lactamase production. E. coli WZ33 and WZ51 exhibited discrepant-level resistance to most clinically frequent used antimicrobials, but still susceptible to trimethoprim/sulfamethoxazole, amikacin, fosfomycin, tigecycline and polymyxin B. E. coli WZ33 and WZ51 were positive for blaNDM-1 determined by PCR and DNA sequencing. Other than blaNDM-1, E. coli WZ33 also harbored blaCTX-M-14 and blaCMY-42, while E. coli WZ51 simultaneously harbored blaSHV-12,blaCTX-M-14 and blaCMY-42. Carbapenem resistance for E. coli WZ51 and WZ33 could not be transferred to E. coli recipients through conjugation, but could be transferred to E. coli recipients by chemical transformation. The EcoR1-digested DNA pattern of plasmids from the transformant of E. coli WZ51 was different from that of E. coli WZ51. MLST showed that E. coli WZ33 and WZ51 belonged to an animal-associated clone (ST167). The present study is the first report of blaNDM-1 carriage in E. coli ST167 isolates and coexistence of blaNDM-1 and blaCMY-42 in same isolate. Systemic surveillance should focus on the dissemination of blaNDM-1 among Enterobacteriaceae, especially E. coli ST167 clone associated with animal infection.
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