Perspective on Emerging Mass Spectrometry Technologies for Comprehensive Lipid Structural Elucidation.
Perspective on Emerging Mass Spectrometry Technologies for Comprehensive Lipid Structural Elucidation.
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DOI：
10.1021/acs.analchem.1c00061
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发表时间：
2021-04-27
影响因子：
7.4
通讯作者：
Prentice BM
中科院分区：
文献类型：
--
作者：
Bonney JR;Prentice BM
脂质和代谢物在许多临床和研究环境中是令人感兴趣的，因为代谢物组学越来越被认为是表型的更动态和灵敏的分子测量。脂质结构的巨大多样性和生物结构-功能关系在各种应用中的重要性使得准确识别成为脂质界具有挑战性但至关重要的研究领域。事实上，脂质化学结构的细微差异可能对细胞代谢和许多疾病病理学产生重要影响。现代质谱所提供的速度、灵敏度和分子特异性使其在脂质组学领域的许多不同仪器平台和实验工作流程中得到广泛采用。然而，通过质谱法对脂质进行明确和完整的结构鉴定仍然具有挑战性。目前正在开发越来越复杂的串联质谱（MS/MS）方法，并将其无缝集成到脂质组学工作流程中，以应对这一挑战。这些方法通常（i）改变被询问的离子类型或（ii）改变解离方法，以改善从MS/MS实验获得的结构信息。在这方面，我们强调了最近的进展，离子类型的改变和离子解离方法的脂质鉴定质谱。本次讨论的目的是从事基本离子化学和技术发展的调查人员，以及脂质组学及其许多应用的从业人员。近年来，技术的快速发展加速并加强了这两个研究团体之间的联系。我们确定了这些新兴方法的共同特点和实用的优点，并讨论了这些方法可能催化脂质结构解析研究的未来方向。
Lipids and metabolites are of interest in many clinical and research settings because it is the metabolome that is increasingly recognized as a more dynamic and sensitive molecular measure of phenotype. The enormous diversity of lipid structures and the importance of biological structure–function relationships in a wide variety of applications makes accurate identification a challenging yet crucial area of research in the lipid community. Indeed, subtle differences in the chemical structures of lipids can have important implications in cellular metabolism and many disease pathologies. The speed, sensitivity, and molecular specificity afforded by modern mass spectrometry has led to its widespread adoption in the field of lipidomics on many different instrument platforms and experimental workflows. However, unambiguous and complete structural identification of lipids by mass spectrometry remains challenging. Increasingly sophisticated tandem mass spectrometry (MS/MS) approaches are now being developed and seamlessly integrated into lipidomics workflows to meet this challenge. These approaches generally either (i) alter the type of ion that is interrogated or (ii) alter the dissociation method in order to improve the structural information obtained from the MS/MS experiment. In this Perspective, we highlight recent advances in both ion type alteration and ion dissociation methods for lipid identification by mass spectrometry. This discussion is aimed to engage investigators involved in fundamental ion chemistry and technology developments as well as practitioners of lipidomics and its many applications. The rapid rate of technology development in recent years has accelerated and strengthened the ties between these two research communities. We identify the common characteristics and practical figures of merit of these emerging approaches and discuss ways these may catalyze future directions of lipid structural elucidation research.
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通过质谱法对组织中脂质生物化学的MALDI成像。
影响因子：
62.1
作者：
Berry, Karin A. Zemski;Hankin, Joseph A.;Barkley, Robert M.;Spraggins, Jeffrey M.;Caprioli, Richard M.;Murphy, Robert C.
通讯作者：
Murphy, Robert C.
影响因子：
46.2
作者：
Brodbelt JS
通讯作者：
Brodbelt JS
影响因子：
7.4
作者：
Bowman, Andrew P.;Blakney, Greg T.;Smith, Donald F.
通讯作者：
Smith, Donald F.
DOI：
10.1007/s13361-016-1359-3
发表时间：
2016-06-01
影响因子：
3.2
作者：
Bu, Jiexun;Fisher, Christine M.;McLuckey, Scott A.
通讯作者：
McLuckey, Scott A.
影响因子：
7.4
作者：
Baba, Takashi;Campbell, J. Larry;Baker, Paul R. S.
通讯作者：
Baker, Paul R. S.