miRNA and mRNA expression profiling identifies members of the miR-200 family as potential regulators of epithelial-mesenchymal transition in pterygium.
miRNA and mRNA expression profiling identifies members of the miR-200 family as potential regulators of epithelial-mesenchymal transition in pterygium.
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DOI：
10.1016/j.exer.2013.07.003
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发表时间：
2013-10
影响因子：
3.4
通讯作者：
Raeder, Sten
中科院分区：
文献类型：
--
作者：
Engelsvold, David H.;Utheim, Tor P.;Olstad, Ole K.;Gonzalez, Pedro;Eidet, Jon R.;Lyberg, Torstein;Troseid, Anne-Marie S.;Dartt, Darlene A.;Raeder, Sten
目前的研究调查了上皮-间质转化（EMT）、组织纤维化和血管生成的microRNA（miRNA）调节因子在人类原发性翼状胬肉中的差异表达。在常规翼状胬肉切除联合自体结膜移植的背景下，对成对翼状胬肉和正常结膜进行全基因组miRNA和mRNA表达谱分析（n=8）。使用定量真实的时间聚合酶链反应（qRT-PCR）来验证先前通过微阵列检测的关键分子的表达。在翼状胬肉中，与正常结膜相比，25种miRNAs和31种mRNAs的表达差异超过2倍。14个miRNAs表达上调（miR-1246、−486、−451、−3172、−3175、−1308、−1972、−143、−211、−665、−1973、− 18 a、143和− 663 b），而11个下调（miR-675、−200b-star、−200a-star、−29b、−200b、−210、−141、−31、−200a、−934和−375）。无监督分层聚类分析表明，miR-200家族成员在翼状胬肉中共表达和下调。对miRNA数据集最重要的分子和细胞功能是细胞发育、细胞生长和增殖以及细胞运动。qRT-PCR证实了检测的16个基因中的15个基因的表达，并显示miR-429在翼状胬肉中下调超过2倍。来自miR-200家族两个簇的四个成员的协同下调（miR-200 a/− 200 b/−429和miR-200 c/−141），已知其调节EMT，并上调预测的靶点和间充质标志物纤连蛋白（FN 1），提示EMT可能在翼状胬肉的发病机制中发挥作用，并可能成为治疗干预的有前途的新靶点翼状胬肉
The current study investigates whether microRNA (miRNA) regulators of epithelial-mesenchymal transition (EMT), tissue fibrosis, and angiogenesis are differentially expressed in human primary pterygium. Genome-wide miRNA and mRNA expression profiling of paired pterygium and normal conjunctiva was performed in the context of conventional excision of pterygium with autotransplantation of conjunctiva (n=8). Quantitative real time polymerase chain reaction (qRT-PCR) was used to validate the expression of key molecules previously detected by microarray. In pterygium, 25 miRNAs and 31 mRNAs were significantly differentially expressed by more than two-fold compared to normal conjunctiva. 14 miRNAs were up-regulated (miR-1246, −486, −451, −3172, −3175, −1308, −1972, −143, −211, −665, −1973, −18a, 143, and −663b), whereas 11 were down-regulated (miR-675, −200b-star, −200a-star, −29b, −200b, −210, −141, −31, −200a, −934, and −375). Unsupervised hierarchical cluster analysis demonstrated that members of the miR-200 family were coexpressed and down-regulated in pterygium. The molecular and cellular functions that were most significant to the miRNA data sets were cellular development, cellular growth and proliferation, and cellular movement. qRT-PCR confirmed the expression of 15 of the 16 genes tested and revealed that miR-429 was down-regulated by more than two-fold in pterygium. The concerted down-regulation of four members from both clusters of the miR-200 family (miR-200a/−200b/−429 and miR-200c/−141), which are known to regulate EMT, and up-regulation of the predicted target and mesenchymal marker fibronectin (FN1), suggest that EMT could potentially play a role in the pathogenesis of pterygium and might constitute promising new targets for therapeutic intervention in pterygium.
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影响因子：
4.4
作者：
Karali, Marianthi;Peluso, Ivana;Banfi, Sandro
通讯作者：
Banfi, Sandro
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
DOI：
10.1073/pnas.0611192104
发表时间：
2007-02-27
影响因子：
11.1
作者：
Kato, Mitsuo;Zhang, Jane;Natarajan, Rama
通讯作者：
Natarajan, Rama
影响因子：
2.3
作者：
Frederikse, Peter H.;Donnelly, Robert;Partyka, Lukasz M.
通讯作者：
Partyka, Lukasz M.
DOI：
10.1126/science.1178178
发表时间：
2010-01-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Lanford RE;Hildebrandt-Eriksen ES;Petri A;Persson R;Lindow M;Munk ME;Kauppinen S;Ørum H
通讯作者：
Ørum H