Overexpression of an auxin receptor OsAFB6 significantly enhanced grain yield by increasing cytokinin and decreasing auxin concentrations in rice panicle.
Overexpression of an auxin receptor OsAFB6 significantly enhanced grain yield by increasing cytokinin and decreasing auxin concentrations in rice panicle.
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DOI：
10.1038/s41598-018-32450-x
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发表时间：
2018-09-19
影响因子：
4.6
通讯作者：
Xing Y
中科院分区：
文献类型：
--
作者：
He Q;Yang L;Hu W;Zhang J;Xing Y
生长素在植物的许多发育过程中发挥着关键作用。生长素信号通路是植物对生长素刺激的一系列反应。然而，许多基因在这一途径中的功能仍然不清楚。TIR/AFB家族基因作为生长素受体，编码F-Box蛋白，直接与生长素结合，通过信号传导途径抑制生长素刺激。本研究通过构建水稻生长素信号F-Box 6（OsAFB 6）超表达株系，使水稻抽穗期明显延迟，每穗颖花数和一次分支数显著增加，最终使水稻产量提高50%。OsAFB 6优先在分生组织活性较强的幼组织中表达，并通过上调OsRR 1和下调Ehd 1的表达水平来抑制开花。过量表达OsAFB 6可延迟抽穗，通过抑制Gn 1a的表达水平增加CK的含量，同时降低幼穗中IAA的含量，从而促进花序分生组织的发育，形成大穗，每穗颖花数多，一次分枝多，籽粒产量高。构建OsAFB 6表达水平不同的品系，为特定地区培育高产品种，充分利用当地的光温资源提供了有利的策略。
Auxin plays critical roles in many developmental processes of plants. The auxin signaling pathway is a series of plant responses to auxin stimuli. However, the functions of many genes in this pathway are still obscure. As auxin receptors, TIR/AFB family genes encode F-Box proteins that directly bind auxin and then transduce the stimulus through the signaling pathway. In this paper, we generated an overexpression line of Auxin-signaling F-Box 6 (OsAFB6) in rice, which largely delayed heading, greatly increased spikelets per panicle and primary branch number and ultimately enhanced grain yield by 50%. OsAFB6 is preferentially expressed in young tissues with stronger meristem activities and suppresses flowering by upregulating OsRR1 and downregulating Ehd1 expression levels. Overexpression of OsAFB6 delayed heading, increased cytokinin (CK) by suppressing the expression level of Gn1a and simultaneously decreased the IAA concentration in the young panicle, which promoted inflorescence meristem development and resulted in large panicles with more spikelets per panicle, primary branches and increased grain yield. It would be a beneficial strategy to generate lines with varied expression levels of OsAFB6 to breed high-yielding cultivars for specific regions that can fully utilize the local sunlight and temperature resources.
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