Up-regulation of cell surface Toll-like receptors on circulating gammadelta T-cells following burn injury.
Up-regulation of cell surface Toll-like receptors on circulating gammadelta T-cells following burn injury.
复制标题
DOI：
10.1016/j.cyto.2008.09.001
复制
发表时间：
2008-12
期刊：
影响因子：
3.8
通讯作者：
Daniel, TanJanika
中科院分区：
文献类型：
--
作者：
Schwacha, Martin G.;Daniel, TanJanika
烧伤与严重炎症和涉及γδ T细胞的先天免疫系统的激活相关。类似地，toll样受体（TLR）与先天性免疫应答的激活相关;然而，尚不清楚严重烧伤后γδ T细胞中TLR表达是否改变。为了研究这一点，使雄性C57 BL/6小鼠经受烧伤（25%TBSA），并且在1天或7天后，分离血液和脾细胞，并进行TLR和其他表型标志物（γδ TCR、αβ TCR、CD 69、CD 120 b）的FACs分析。在烧伤后24小时观察到循环γδ T细胞的数量显著增加（14%对4%），并且这些细胞中表达TLR-2的百分比更高。TLR-4的表达也增加烧伤后，但程度较轻。TLR表达的这些变化与γδ T细胞中CD 69或CD 120 b表达的改变无关。γδ T细胞的动员和TLR表达的增加是短暂的，因为在烧伤后7天或来自循环或脾脏的γδ T细胞中表型变化不明显。烧伤后未观察到αβ T细胞TLR表达的增加。总之，在烧伤损伤后24小时，观察到具有增加的TLR表达的γδ T细胞的动员。这一发现表明，这种独特的T细胞群在对损伤的先天免疫反应中至关重要，可能是通过TLR识别危险信号。
Burn injury is associated with profound inflammation and activation of the innate immune system involving γδ T-cells. Similarly, toll-like receptors (TLR) are associated with activation of the innate immune response; however, it is unclear whether TLR expression is altered in γδ T-cells after major burn injury. To study this, male C57BL/6 mice were subjected to burn injury (25% TBSA) and 1 or 7 days thereafter, blood and spleen cells were isolated and subjected to FACs analysis for TLRs and other phenotypic markers (γδ TCR, αβ TCR, CD69, CD120b). A marked increase in the number of circulating γδ T-cells was observed at 24 hr. post-burn (14% vs. 4%) and a higher percentage of these cells expressed TLR-2. TLR-4 expression was also increased post-burn, but to a lesser degree. These changes in TLR expression were not associated with altered CD69 or CD120b expression in γδ T-cells. The mobilization of, and increased TLR expression in, γδ T-cells was transient, as phenotypic changes were not evident at 7 days post-burn or in γδ T-cells from the circulation or spleen. The increases in TLR expression were not observed in αβ T-cells after burn injury. In conclusion, 24 hr. after burn injury mobilization of γδ T-cells with increased TLR expression was observed. This finding suggests that this unique T-cell population is critical in the innate immune response to injury, possibly through the recognition of danger signals by TLRs.
登录
查看更多内容
影响因子：
3.1
作者：
Daniel, TanJanika;Thobe, Ljoern M.;Schwacha, Martin G.
通讯作者：
Schwacha, Martin G.
影响因子：
5.6
作者：
Daniel, Tanjanika;Alexander, Michelle;Schwacha, Martin G.
通讯作者：
Schwacha, Martin G.
影响因子：
16.8
作者：
Ferrarini, M;Ferrero, E;Zocchi, MR
通讯作者：
Zocchi, MR
影响因子：
5.5
作者：
Maung, AA;Fujimi, S;Lederer, JA
通讯作者：
Lederer, JA
影响因子：
2.7
作者：
LIU, Y;HSU, BX
通讯作者：
HSU, BX