Deep sequencing-based transcriptome analysis of the oil-bearing plant Physic Nut (Jatropha curcas L.) under cold stress
Deep sequencing-based transcriptome analysis of the oil-bearing plant Physic Nut (Jatropha curcas L.) under cold stress
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基于深度测序的冷胁迫下油料植物麻疯树转录组分析
DOI：
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发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Ming Gong
中科院分区：
文献类型：
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作者：
Haibo Wang;Zhurong Zou;Shasha Wang;Ming Gong
现为小桐子Jatropha curcas L.作为一种重要的可再生生物能源植物，旨在防止化石燃料可能带来的能源危机，在科学和商业领域获得了越来越多的关注。然而，目前对该生物质燃料灌木抗寒性的研究尚不多见，对其遗传改良和生物技术应用提供的信息有限。在这项工作中，新开发的Illumina Hiseq™ 2000 RNA-seq是一种深度高通量测序方法，优先用于J. curcas的抗寒性相关转录组分析。从测序结果中，获得的非冗余序列的总长度为4，960，092，780 bp，由干净数据组装的106，749个重叠群和45，251个单基因组成。共有35，791个单一基因（79.09%）可以注释到众多数据库（Nr，Swiss-Prot，GO，COG，KEGG）进行功能分类。通过数据库比对和EST扫描预测，分别得到33、361和912个完整或部分CDS。其中27，293个单基因可分为GO的61个功能组，11，887个推测的蛋白质可分为至少25个分子家族，18，787个可能参与KEGG中大约128条已知的代谢或信号通路。本研究提供了一个全面的抗寒性转录组分析的J. curcas与显着更多的EST序列的数量比以前所有相关的存款在公共数据库。这些结果使我们能够在该序列库中破译与编码耐寒性相关的关键基因。
Nowadays Jatropha curcas L. has gained an increased attention in scientific and commercial fields as an important renewable bioenergy plant, aiming to prevent the possible energy crisis of fossil fuels. However, the studies on cold resistance of this biofuel shrub are still scarce, giving limited information for its genetic improvement and other biotechnological applications. In this work, the newly developed Illumina Hiseq™ 2000 RNA-seq, which is a deep high-throughput sequencing approach, preferentially was used for cold-resistance related transcriptome analysis of J. curcas. From the sequencing results, the total length of non-redundant sequences obtained was 4,960,092,780 bp, consisting of 106,749 contigs and 45,251 unigenes assembled by clean data. A total of 35,791 unigenes (79.09%) can be annotated to numerous databases (Nr, Swiss-Prot, GO, COG, KEGG) for functional classification. The 33,361 and 912 complete or partial CDSs are deduced by database alignment and ESTscan prediction, respectively. Among these unigenes, 27,293 can be categorized into 61 functional groups of GO, 11,887 of COG-annotated putative proteins were classified functionally into at least 25 molecular families, 18,787 were possibly involved in approximately 128 known metabolic or signaling pathways in KEGG. This study provided a comprehensive cold-resistance transcriptome analysis of J. curcas with remarkably more number of EST sequences than all previous relevant deposits in public databases. The results allowed us to decipher the key genes related to those coding for cold tolerance in this sequence library.
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影响因子：
5.8
作者：
Tong, Mark Y.;Cassa, Christopher A.;Kohane, Isaac S.
通讯作者：
Kohane, Isaac S.
DOI：
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发表时间：
2005
期刊：
--
影响因子：
--
作者：
Ana Conesa;Stefan Götz;Juan Miguel García-Gómez;Javier Terol;Manuel Talón;Montserrat Robles
通讯作者：
Ana Conesa;Stefan Götz;Juan Miguel García-Gómez;Javier Terol;Manuel Talón;Montserrat Robles
来自麻风树的一种新型甜菜碱醛脱氢酶基因，编码一种与适应环境胁迫有关的酶
影响因子：
5.2
作者：
Tang, Lin;Wu, Jun;Zhang, Fu-Li;Chen, Fang;Xu, Ying;Jiang, Lu-Ding;Jia, Yong-Rong;Wang, Ying-Chun;Wang, Sheng-Hua;Gao, Shun;Niu, Bei
通讯作者：
Niu, Bei
影响因子：
4.4
作者：
Natarajan P;Parani M
通讯作者：
Parani M
影响因子：
5.1
作者：
Mingjuan Tang;Jingwen Sun;Yun Liu;Fan Chen;S. Shen
通讯作者：
Mingjuan Tang;Jingwen Sun;Yun Liu;Fan Chen;S. Shen