Interactions of WASp, myosin-I, and verprolin with Arp2/3 complex during actin patch assembly in fission yeast.
Interactions of WASp, myosin-I, and verprolin with Arp2/3 complex during actin patch assembly in fission yeast.
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DOI：
10.1083/jcb.200502053
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发表时间：
2005-08-15
期刊：
影响因子：
--
通讯作者：
Pollard TD
中科院分区：
文献类型：
--
作者：
Sirotkin V;Beltzner CC;Marchand JB;Pollard TD
酵母肌动蛋白斑块是在细胞生长部位形成的动态结构，被认为在胞吞作用中起作用。我们使用生物化学分析和活细胞成像来研究裂殖酵母裂殖酵母中肌动蛋白补丁的组装。贴片组装通过两条平行的途径进行：一条依赖于WASp Wsp 1 p和verprolin Vrp 1 p，另一条依赖于1类肌球蛋白Myo 1 p以激活肌动蛋白相关蛋白2/3（Arp 2/3）复合物。Wsp 1 p通过常规机制激活Arp 2/3复合物，导致分支丝。Myo 1 p是一种较弱的Arp 2/3复合物激活剂，可产生不稳定的分支，并通过verprolin增强。在体内贴片组装过程中，Wsp 1 p和Vrp 1 p首先独立于Myo 1 p到达。Arp 2/3复合物与新生激活剂斑块在6-9秒内缔合，同时保持静止。在达到最大浓度后，Arp 2/3复合物斑块随着激活蛋白的解离而向心移动。斑块形成的遗传依赖性表明，斑块形成涉及Myo 1 p和Wsp 1 p/Vrp 1 p途径之间的串扰。
Yeast actin patches are dynamic structures that form at the sites of cell growth and are thought to play a role in endocytosis. We used biochemical analysis and live cell imaging to investigate actin patch assembly in fission yeast Schizosaccharomyces pombe. Patch assembly proceeds via two parallel pathways: one dependent on WASp Wsp1p and verprolin Vrp1p converges with another dependent on class 1 myosin Myo1p to activate the actin-related protein 2/3 (Arp2/3) complex. Wsp1p activates Arp2/3 complex via a conventional mechanism, resulting in branched filaments. Myo1p is a weaker Arp2/3 complex activator that makes unstable branches and is enhanced by verprolin. During patch assembly in vivo, Wsp1p and Vrp1p arrive first independent of Myo1p. Arp2/3 complex associates with nascent activator patches over 6–9 s while remaining stationary. After reaching a maximum concentration, Arp2/3 complex patches move centripetally as activator proteins dissociate. Genetic dependencies of patch formation suggest that patch formation involves cross talk between Myo1p and Wsp1p/Vrp1p pathways.
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