High dose combination pertussis toxin induces autoimmune inner ear disease in Sprague-Dawley rats
High dose combination pertussis toxin induces autoimmune inner ear disease in Sprague-Dawley rats
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高剂量组合百日咳毒素诱发斯普拉格-道利大鼠自身免疫性内耳疾病
DOI：
10.3109/00016489.2011.552525
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发表时间：
2011-06
影响因子：
1.4
通讯作者：
Wang, D.-Y.
中科院分区：
文献类型：
--
作者：
Huang, X.;Kong, W.-J.;Ding, G.-F.;Wang, D.-Y.
摘要结论：用大剂量百日咳毒素联合应用建立了一种较好的自身免疫性内耳病（AIED）动物模型。本研究还表明内耳特异性抗原可参与自身免疫反应。细胞介导的免疫损伤在AIED的发生中起重要作用，至少在早期是如此。目的：本研究的目的是开发一种更合适的大鼠模型，该模型与AIED的病理生理过程更相似。方法：96只雌性SD大鼠随机分为4组。分别用粗制内耳抗原/完全弗氏佐剂（CIEAg/CFA）皮下免疫、500 ng百日咳毒素（PT）腹腔注射、CIEAg/CFA+PT腹腔注射、磷酸盐缓冲液（PBS）单独注射。检查听功能、内耳组织病理学和自身抗体。结果如下：CIEAg/CFA+PT组动物的听性脑干反应（ABR）阈值和细胞浸润平均评分的时间过程中表现出显着差异。免疫后，毛细胞缺失、螺旋神经节细胞变性、内淋巴积水和自身抗体均出现。在任何其他动物组中，ABR阈值或组织病理学均无显著差异。
Abstract Conclusion: A better animal model of autoimmune inner ear disease (AIED) in Sprague-Dawley rats has been developed by combination with high dose of pertussis toxin. This study also indicated that inner ear-specific antigens can be involved in autoimmune reactions. Cell-mediated immune injury can play an important role in the induction of AIED, at least in the earlier stage. Objectives: The purpose of this study was to develop a more suitable rat model that demonstrated closer resemblance to the pathophysiological process in AIED. Methods: Ninety-six female Sprague-Dawley rats were divided into four groups. They were subcutaneously immunized with crude inner ear antigen/complete Freund's adjuvant (CIEAg/CFA), or intraperitoneal injection of 500 ng pertussis toxin (PT), or injection of CIEAg/CFA+PT, or phosphate-buffered saline (PBS) alone. The auditory function, histopathology of the inner ear, and autoantibodies were examined. Results: Significant differences in the time course of auditory brainstem response (ABR) threshold and mean score of cellular infiltration were demonstrated in the CIEAg/CFA+PT group of animals. Missing hair cells, degeneration of the spiral ganglion cells, endolymphatic hydrops, and autoantibodies were all noted after immunization. There were no significant differences in ABR threshold or histopathology in any other group of animals.
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影响因子：
1.4
作者：
MCCABE, BF
通讯作者：
MCCABE, BF
DOI：
10.1288/00005537-198902000-00011
发表时间：
1989-02
期刊：
The Laryngoscope
影响因子：
--
作者：
A. Soliman
通讯作者：
A. Soliman
DOI：
10.1177/000348940411300512
发表时间：
2004-05
期刊：
Annals of Otology, Rhinology & Laryngology
影响因子：
--
作者：
J. García-Berrocal;A. Trinidad;R. B. de la Fuente;R. Ramírez‐Camacho;M. Zurita;D. Lobo
通讯作者：
J. García-Berrocal;A. Trinidad;R. B. de la Fuente;R. Ramírez‐Camacho;M. Zurita;D. Lobo
影响因子：
2.5
作者：
Hoistad, DL;Schachern, PA;Paparella, MM
通讯作者：
Paparella, MM
影响因子：
4.3
作者：
Denkinger, Claudia M.;Denkinger, Michael D.;Forsthuber, Thomas G.
通讯作者：
Forsthuber, Thomas G.