Effect of Stratification on Surface Properties of Corneal Epithelial Cells.
Effect of Stratification on Surface Properties of Corneal Epithelial Cells.
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分层对角膜上皮细胞表面性质的影响。
DOI：
10.1167/iovs.15-17468
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发表时间：
2015
影响因子：
4.4
通讯作者：
Murphy,ChristopherJ
中科院分区：
文献类型：
--
作者：
Yáñez-Soto,Bernardo;Leonard,BrianC;Raghunathan,VijayKrishna;Abbott,NicholasL;Murphy,ChristopherJ
目的：研究永生化人角膜上皮细胞系(HTCEpi)中粘蛋白表达对细胞表面润湿性、接触角和表面异质性的影响。然后用分层培养基代换成诱导粘蛋白生物合成的培养液。用定量聚合酶链式反应和Western blotting分析粘蛋白表达谱。采用两不混相液体法测量接触角，并通过倾斜仪器和记录前进和后退接触角来评估接触角滞后。结果：hTCEpi细胞表达3种主要的眼粘蛋白(MUC1、MUC4和MUC16)，在层积过程的第1~3天达到最大值。在分层后，单元导致接触角滞后显著增加，这表明由地形和/或化学功能定义的空间离散和不均匀分布的表面特征的发展。虽然原子力显微镜测量显示细胞表面没有形成明显的表面特征，但我们观察到表面化学不均一性显著增加。结论：角膜上皮表面化学不均一性可能通过“钉扎”细胞表面和泪膜之间的接触线而影响泪膜的动态行为。改造角膜上皮的表面特性可能会导致干眼症的新治疗方法。
Purpose: The purpose of this study was to determine the influence of mucin expression in an immortalized human corneal epithelial cell line (hTCEpi) on the surface properties of cells, such as wettability, contact angle, and surface heterogeneity.Methods: hTCEpi cells were cultured to confluence in serum-free medium. The medium was then replaced by stratification medium to induce mucin biosynthesis. The mucin expression profile was analyzed using quantitative PCR and Western blotting. Contact angles were measured using a two-immiscible liquid method, and contact angle hysteresis was evaluated by tilting the apparatus and recording advancing and receding contact angles. The spatial distribution of mucins was evaluated with fluorescently labeled lectin.Results: hTCEpi cells expressed the three main ocular mucins (MUC1, MUC4, and MUC16) with a maximum between days 1 and 3 of the stratification process. Upon stratification, cells caused a very significant increase in contact angle hysteresis, suggesting the development of spatially discrete and heterogeneously distributed surface features, defined by topography and/or chemical functionality. Although atomic force microscopy measurements showed no formation of appreciable topographic features on the surface of the cells, we observed a significant increase in surface chemical heterogeneity.Conclusions: The surface chemical heterogeneity of the corneal epithelium may influence the dynamic behavior of tear film by “pinning” the contact line between the cellular surface and aqueous tear film. Engineering the surface properties of corneal epithelium could potentially lead to novel treatments in dry eye disease.
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DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen
影响因子：
2.8
作者：
Watanabe, Hitoshi
通讯作者：
Watanabe, Hitoshi
影响因子：
3.4
作者：
J. Tiffany
通讯作者：
J. Tiffany
干眼症人结膜上皮中粘蛋白的改变。
DOI：
--
发表时间：
1998
期刊：
Investigative ophthalmology & visual science.
影响因子：
--
作者：
Danjo,Y;Watanabe,H;Tisdale,AS;George,M;Tsumura,T;Abelson,MB;Gipson,IK
通讯作者：
Gipson,IK
影响因子：
4.4
作者：
Gipson, IK;Spurr-Michaud, S;Russo, CL
通讯作者：
Russo, CL