An organ-specific role for ethylene in rose petal expansion during dehydration and rehydration.
An organ-specific role for ethylene in rose petal expansion during dehydration and rehydration.
复制标题
乙烯在脱水和再水合过程中玫瑰花瓣膨胀中的器官特异性作用
DOI：
10.1093/jxb/ert092
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发表时间：
2013-05
影响因子：
6.9
通讯作者：
Gao J
中科院分区：
文献类型：
--
作者：
Liu D;Liu X;Meng Y;Sun C;Tang H;Jiang Y;Khan MA;Xue J;Ma N;Gao J
脱水是切花在运输过程中造成巨大损失的主要因素。在本研究中，脱水抑制了花瓣细胞的扩张，并导致切花玫瑰的花朵不规则，模仿乙烯处理的花朵。在五个花器官中，脱水显著提高了萼片中的乙烯产生，而复水导致雌蕊和萼片中的乙烯水平迅速升高。在5个乙烯生物合成酶基因（RhACS 1 -5）中，RhACS 1和RhACS 2的表达受两种花器官脱水和复水的诱导。沉默RhACS 1和RhACS 2显着抑制脱水和复水诱导的乙烯在萼片和雌蕊。这减弱了脱水对花瓣细胞扩张的抑制作用。由RhACS 1和RhACS 2启动子驱动的β-葡萄糖醛酸酶活性在转基因拟南芥的萼片、雌蕊和雄蕊中显著诱导，但在花瓣中不诱导。这进一步支持了这两个基因的器官特异性诱导。在5个玫瑰乙烯受体基因（RhETR 1 -5）中，RhETR 3的表达主要受花瓣脱水和复水的诱导。RhETR 3沉默明显加重了脱水对花瓣细胞扩张的抑制作用。然而，没有观察到RhETR 3沉默的花和RhETR基因沉默的花之间的效果的显著差异。此外，RhETR基因沉默广泛改变了21个细胞扩增相关的下游基因的表达响应乙烯。这些结果表明，诱导乙烯生物合成的脱水进行器官特异性的方式，表明乙烯可以作为一个调解人在脱水引起的抑制玫瑰花瓣中的细胞扩张。
Dehydration is a major factor resulting in huge loss from cut flowers during transportation. In the present study, dehydration inhibited petal cell expansion and resulted in irregular flowers in cut roses, mimicking ethylene-treated flowers. Among the five floral organs, dehydration substantially elevated ethylene production in the sepals, whilst rehydration caused rapid and elevated ethylene levels in the gynoecia and sepals. Among the five ethylene biosynthetic enzyme genes (RhACS1–5), expression of RhACS1 and RhACS2 was induced by dehydration and rehydration in the two floral organs. Silencing both RhACS1 and RhACS2 significantly suppressed dehydration- and rehydration-induced ethylene in the sepals and gynoecia. This weakened the inhibitory effect of dehydration on petal cell expansion. β-glucuronidase activity driven by both the RhACS1 and RhACS2 promoters was dramatically induced in the sepals, pistil, and stamens, but not in the petals of transgenic Arabidopsis. This further supports the organ-specific induction of these two genes. Among the five rose ethylene receptor genes (RhETR1–5), expression of RhETR3 was predominantly induced by dehydration and rehydration in the petals. RhETR3 silencing clearly aggravated the inhibitory effect of dehydration on petal cell expansion. However, no significant difference in the effect between RhETR3-silenced flowers and RhETR-genes-silenced flowers was observed. Furthermore, RhETR-genes silencing extensively altered the expression of 21 cell expansion-related downstream genes in response to ethylene. These results suggest that induction of ethylene biosynthesis by dehydration proceeds in an organ-specific manner, indicating that ethylene can function as a mediator in dehydration-caused inhibition of cell expansion in rose petals.
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影响因子：
7.4
作者：
ADATO, I;GAZIT, S
通讯作者：
GAZIT, S
DOI：
10.1360/062004-37
发表时间：
2005-10-01
期刊：
SCIENCE IN CHINA SERIES C-LIFE SCIENCES
影响因子：
--
作者：
Ma, N;Cai, L;Gao, JP
通讯作者：
Gao, JP
影响因子：
7.4
作者：
MORGAN, PW;HE, CJ;DEPROFT, MP
通讯作者：
DEPROFT, MP
影响因子：
4.2
作者：
Beltrano, J;Montaldi, E;Carbone, A
通讯作者：
Carbone, A
影响因子：
7.2
作者：
Liu, YL;Schiff, M;Dinesh-Kumar, SP
通讯作者：
Dinesh-Kumar, SP