Comparative studies of mammalian acid lipases: Evidence for a new gene family in mouse and rat (Lipo).
Comparative studies of mammalian acid lipases: Evidence for a new gene family in mouse and rat (Lipo).
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DOI：
10.1016/j.cbd.2010.05.004
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发表时间：
2010-09
影响因子：
3
通讯作者：
VandeBerg, John L.
中科院分区：
文献类型：
--
作者：
Holmes, Roger S.;Cox, Laura A.;VandeBerg, John L.
至少有 6 个哺乳动物酸性脂肪酶家族 (E.C. 3.1.1.-) 催化体内甘油三酯的水解，称为 LIPA（溶酶体）、LIPF（胃）、LIPJ（睾丸）和 LIPK、LIPM 和 LIPN（表皮），属于 AB 水解酶超家族。在这项研究中，利用计算机方法利用来自多个哺乳动物基因组项目的数据来预测酸性脂肪酶基因和编码蛋白质的氨基酸序列、二级和三级结构以及基因位置。哺乳动物酸性脂肪酶基因位于所检查的 8 个哺乳动物基因组的基因簇内，包括人类 (Homo sapiens)、黑猩猩 (Pons troglodytes)、恒河猴 (Macacca mulatta)、小鼠 (Mus musculus)、大鼠 (Rattusnorvegicus)、牛 (Bos taurus)、马 (Equus caballus) 和狗 (Canis familaris)，每个含有9个编码外显子。人和小鼠酸性脂肪酶具有 44-87% 的序列同一性，并且与之前报道的人胃脂肪酶 (LIPF) 的关键氨基酸残基和预测的二级和三级结构的保守性表现出序列比对和同一性。据报道，小鼠和大鼠基因组中存在一个新的酸性脂肪酶基因家族，称为 Lipo。小鼠酸性脂肪酶基因的 mRNA 组织表达存在差异，Lipa 显示广泛的组织表达，而其他基因在消化道 (Lipf)、唾液腺 (Lipo) 和表皮组织 (Lipk、Lipm 和 Lipn) 中的组织表达更为有限。对哺乳动物酸性脂肪酶基因家族的系统发育分析表明，这些基因是真兽类哺乳动物进化之前基因复制事件的产物，源自祖先脊椎动物 LIPA 基因，该基因存在于青蛙热带爪蟾中。
At least six families of mammalian acid lipases (E.C. 3.1.1.-) catalyse the hydrolysis of triglycerides in the body, designated as LIPA (lysosomal), LIPF (gastric), LIPJ (testis) and LIPK, LIPM and LIPN (epidermal), which belong to the AB hydrolase superfamily. In this study, in silico methods were used to predict the amino acid sequences, secondary and tertiary structures, and gene locations for acid lipase genes and encoded proteins using data from several mammalian genome projects. Mammalian acid lipase genes were located within a gene cluster for each of the 8 mammalian genomes examined, including human (Homo sapiens), chimpanzee (Pons troglodytes), rhesus monkey (Macacca mulatta), mouse (Mus musculus), rat (Rattus norvegicus), cow (Bos taurus), horse (Equus caballus) and dog (Canis familaris), with each containing 9 coding exons. Human and mouse acid lipases shared 44-87% sequence identity and exhibited sequence alignments and identities for key amino acid residues and conservation of predicted secondary and tertiary structures with those previously reported for human gastric lipase (LIPF). Evidence for a new family of acid lipase genes is reported for mouse and rat genomes, designated as Lipo. Mouse acid lipase genes are subject to differential mRNA tissue expression, with Lipa showing wide tissue expression, while others have a more restricted tissue expression in the digestive tract (Lipf), salivary gland (Lipo) and epidermal tissues (Lipk, Lipm and Lipn). Phylogenetic analyses of the mammalian acid lipase gene families suggested that these genes are products of gene duplication events prior to eutherian mammalian evolution and derived from an ancestral vertebrate LIPA gene, which is present in the frog, Xenopus tropicalis.
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影响因子：
56.9
作者：
Carninci, P;Kasukawa, T;Hayashizaki, Y
通讯作者：
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影响因子：
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作者：
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通讯作者：
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作者：
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影响因子：
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作者：
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通讯作者：
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