Effects of Preservation and Propagation Methodology on Microcosms Derived from the Oral Microbiome.
Effects of Preservation and Propagation Methodology on Microcosms Derived from the Oral Microbiome.
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DOI：
10.3390/microorganisms10112146
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发表时间：
2022-10-29
期刊：
影响因子：
4.5
通讯作者：
Chen IA
中科院分区：
文献类型：
--
作者：
Zhou B;Mobberley J;Shi K;Chen IA
创建具有可重复成分的口腔微生物对于开发口腔微生物组模型系统非常重要。然而，口腔微生物群因人而异。为了从来自不同个体的接种物中获得可重复的组成，我们测试了冷藏和在特定培养基中培养的选择性条件是否会产生可重复的群落组成，尽管存在个体差异。在这项初步研究中，我们收集了三个个体的牙菌斑刮屑，在厌氧条件下接种培养基，并表征了冷藏和随后在液体培养基中繁殖后的细菌群落组成。提取收获的培养物并通过 16S rRNA 基因扩增子测序和 mothur 管道确定细菌组成。我们的结果表明，三分之二的样本聚集成特定的成分类型（此处称为“吸引子”）。此外，来自第三个体的样本在体外繁殖后可以采用这种吸引子成分类型，尽管其原始成分并未表现出这种类型。这些结果表明，尽管来自不同个体的牙菌斑样本存在差异，但简单的选择性环境可以帮助创建可重复的微观世界。这些发现说明了从人类口腔微生物组创建可重复的微观世界时需要考虑的重要参数。
The creation of oral microcosms with reproducible composition is important for developing model systems of the oral microbiome. However, oral microbiomes vary substantially across individuals. To derive a reproducible composition from inocula sourced from different individuals, we tested whether selective conditions from cold storage and culturing in defined media would generate a reproducible community composition despite individual variations. In this pilot study, we collected dental plaque scrapings from three individuals, inoculated media under anaerobic conditions, and characterized the bacterial community compositions after cold storage and subsequent propagation in liquid media. Harvested cultures were extracted and bacterial composition was determined by 16S rRNA gene amplicon sequencing and the mothur pipeline. Our results show that samples from two out of three individuals clustered into a specific compositional type (termed “attractor” here). In addition, the samples from the third individual could adopt this attractor compositional type after propagation in vitro, even though its original composition did not display this type. These results indicate that simple selective environments could help create reproducible microcosms despite variation among dental plaque samples sourced from different individuals. The findings illustrate important parameters to consider for creating reproducible microcosms from the human oral microbiome.
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影响因子：
15.5
作者：
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