Genetic relatedness of multidrug resistant Escherichia coli isolated from humans, chickens and poultry environments.
Genetic relatedness of multidrug resistant Escherichia coli isolated from humans, chickens and poultry environments.
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从人、鸡和家禽环境中分离的多重耐药大肠杆菌的遗传相关性。
DOI：
10.1186/s13756-021-00930-x
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发表时间：
2021-03-23
影响因子：
5.5
通讯作者：
Thakur S
中科院分区：
文献类型：
--
作者：
Aworh MK;Kwaga JKP;Hendriksen RS;Okolocha EC;Thakur S
动物生产中抗菌剂使用不当导致食源性致病菌产生抗菌素耐药性(AMR)。确实发生了AMR食源性病原体从水库，特别是鸡向人群传播的情况。最近，我们报道了职业暴露是家禽从业人员多药耐药(MDR)大肠埃希氏菌(E.coli)的危险因素。在这里，我们确定了从尼日利亚阿布贾的家禽工作者、鸡和家禽环境中分离的多药耐药大肠杆菌的流行率和遗传相关性。进行这项研究是为了弥补尼日利亚AMR情况分析所确定的差距。我们在家禽工人、鸡和家禽养殖场/活禽市场(LBM)环境中进行了一项横断面研究。对这些分离株进行了表型测试，用全基因组测序(WGS)和电子多位点序列类型(MLST)对其进行了表型和基因特征鉴定。我们进行了系统发育单核苷酸多态性(SNPs)分析，以确定分离株之间的亲缘关系和克隆性。在429份样本中，共有115份(26.8%)对大肠杆菌呈阳性反应。在这些菌株中，110株能够进行表型和基因特征鉴定。本次筛选包括来自家禽从业人员的47株(42.7%)，来自鸡群的36株(32.7%)，来自家禽养殖场或LBM环境的27株(24.5%)。总体而言，101株(91.8%)分离株对至少三种药物类别具有耐药性。耐药率较高的有四环素(n = 102；92.7%)、甲氧苄氨嘧啶/磺胺甲恶唑(n = 93；84.5%)、链霉素(n = 87；79.1%)和氨苄西林(n = 88；80%)。在环境分离株中检测到两个携带在IncX4质粒上的质粒介导的粘菌素基因-mcr-1.1。最常见的序列类型是ST-155(n = 8)、ST-48(n = 8)和ST-10(n = 6)。来自同一农场的两个人源和环境源分离株的SNPs差异为6161，共享一个新的ST。分离株具有相似的AMR基因和质粒复制子。耐多药大肠杆菌分离株在家禽工作者、家禽和家禽养殖场/LBM环境中普遍存在。在两个分离株中出现带有新ST的多药耐药大肠埃希菌可能是由质粒介导的。主管当局应执行AMR法规，以确保谨慎使用抗菌剂，以限制食物链传播的风险。网上版载有补充材料，可在10.1186/s13756-021-00930-x查阅。
Inappropriate use of antimicrobial agents in animal production has led to the development of antimicrobial resistance (AMR) in foodborne pathogens. Transmission of AMR foodborne pathogens from reservoirs, particularly chickens to the human population does occur. Recently, we reported that occupational exposure was a risk factor for multidrug-resistant (MDR) Escherichia coli (E. coli) among poultry-workers. Here we determined the prevalence and genetic relatedness among MDR E. coli isolated from poultry-workers, chickens, and poultry environments in Abuja, Nigeria. This study was conducted to address the gaps identified by the Nigerian AMR situation analysis. We conducted a cross-sectional study among poultry-workers, chickens, and poultry farm/live bird market (LBM) environments. The isolates were tested phenotypically for their antimicrobial susceptibility profiles, genotypically characterized using whole-genome sequencing (WGS) and in silico multilocus sequence types (MLST). We conducted a phylogenetic single nucleotide polymorphism (SNPs) analysis to determine relatedness and clonality among the isolates. A total of 115 (26.8%) out of 429 samples were positive for E. coli. Of these, 110 isolates were viable for phenotypic and genotypic characterization. The selection comprised 47 (42.7%) isolates from poultry-workers, 36 (32.7%) from chickens, and 27 (24.5%) from poultry-farm or LBM environments. Overall, 101 (91.8%) of the isolates were MDR conferring resistance to at least three drug classes. High frequency of resistance was observed for tetracycline (n = 102; 92.7%), trimethoprim/sulfamethoxazole (n = 93; 84.5%), streptomycin (n = 87; 79.1%) and ampicillin (n = 88; 80%). Two plasmid-mediated colistin genes—mcr-1.1 harboured on IncX4 plasmids were detected in environmental isolates. The most prevalent sequence types (ST) were ST-155 (n = 8), ST-48 (n = 8) and ST-10 (n = 6). Two isolates of human and environmental sources with a SNPs difference of 6161 originating from the same farm shared a novel ST. The isolates had similar AMR genes and plasmid replicons. MDR E.coli isolates were prevalent amongst poultry-workers, poultry, and the poultry farm/LBM environment. The emergence of MDR E. coli with novel ST in two isolates may be plasmid-mediated. Competent authorities should enforce AMR regulations to ensure prudent use of antimicrobials to limit the risk of transmission along the food chain. The online version contains supplementary material available at 10.1186/s13756-021-00930-x.
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DOI：
10.3390/molecules23040795
发表时间：
2018-03-30
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Manyi-Loh C;Mamphweli S;Meyer E;Okoh A
通讯作者：
Okoh A
影响因子：
11.8
作者：
Manges, Amee R.;Johnson, James R.
通讯作者：
Johnson, James R.
影响因子：
4.9
作者：
Madec, Jean-Yves;Haenni, Marisa;Gassilloud, Benoit
通讯作者：
Gassilloud, Benoit
影响因子：
3.7
作者：
Kaas RS;Leekitcharoenphon P;Aarestrup FM;Lund O
通讯作者：
Lund O
影响因子：
3.7
作者：
Aworh, Mabel Kamweli;Kwaga, Jacob;Thakur, Siddhartha
通讯作者：
Thakur, Siddhartha