Gold nanoparticle-enhanced capillary electrophoresis-chemiluminescence assay of trace uric acid.
Gold nanoparticle-enhanced capillary electrophoresis-chemiluminescence assay of trace uric acid.
复制标题
金纳米颗粒增强的毛细血管电泳 - 化石测定痕量尿酸。
DOI：
10.1002/elps.200900115
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发表时间：
2009-08
期刊：
影响因子：
2.9
通讯作者：
Liu, Yi-Ming
中科院分区：
文献类型：
--
作者：
Zhao, Shulin;Lan, Xuehua;Liu, Yi-Ming
建立了纳米金增强毛细管电泳-化学发光检测血清中尿酸的方法。在毛细管电泳运行缓冲液中加入纳米金催化鲁米诺和过氧化氢的柱后化学发光反应，实现高效的化学发光。负峰的产生是由于对从电泳毛细管中洗脱的尿酸的化学发光的抑制作用。在2.5 × 10−7 ~ 1.0 × 10−5 M范围内，化学发光强度的降低与尿酸浓度成正比。检测限为4.6 × 10−8 M尿酸。用该方法分析了10份人血清样品。健康受试者（n=5）的血清尿酸水平范围为204 - 324 μM，糖尿病患者（n = 5）的血清尿酸水平范围为464 - 497 μM。两组比较有显著性差异（p < 0.05）。结果表明，所提出的检测方法在糖尿病等疾病的快速初步诊断中具有潜在的应用价值。
A sensitive method based on gold nanoparticle-enhanced capillary electrophoresis-chemiluminescence detection was developed for quantifying uric acid in serum. In this work, gold nanoparticles were added into the running buffer of capillary electrophoresis to catalyze the post-column chemiluminescence reaction between luminol and hydrogen peroxide, achieving highly efficient chemiluminescence emission. Negative peaks were produced due to the inhibitory effects on chemiluminescence emission from uric acid eluted from the electrophoretic capillary. The decrease in chemiluminescence intensity was proportional to the concentration of uric acid in the range of 2.5 × 10−7 ~ 1.0 × 10−5 M. Detection limit was 4.6 × 10−8 M uric acid. Ten human serum samples were analyzed by the presented method. Serum level of uric acid was found to be in the range from 204 to 324 μM for healthy subjects (n=5), and from 464 to 497 μM for diabetic patients (n=5). The two groups were significantly different (p < 0.05). The results suggested a potential application of the proposed assay in rapid primary diagnosis of diseases such as diabetes.
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DOI：
10.1016/j.jchromb.2007.07.035
发表时间：
2007-10-01
影响因子：
3
作者：
Dai, Xinhua;Fang, Xiang;Xu, Bei
通讯作者：
Xu, Bei
影响因子：
4.3
作者：
Guan, YQ;Chu, QC;Ye, JN
通讯作者：
Ye, JN
影响因子：
4.3
作者：
Fang, Bin;Jiao, Shoufeng;Tao, Haisheng
通讯作者：
Tao, Haisheng
影响因子：
7.4
作者：
Yu, Cheng-Ju;Su, Chih-Lin;Tseng, Wei-Lung
通讯作者：
Tseng, Wei-Lung
影响因子：
1.6
作者：
Zuo, Yuegang;Wang, Chengjun;Ruelos, Vanessa C.
通讯作者：
Ruelos, Vanessa C.