Molecular characterization of direct target genes and cis-acting consensus recognized by quorum-sensing regulator AphA in Vibrio parahaemolyticus.
Molecular characterization of direct target genes and cis-acting consensus recognized by quorum-sensing regulator AphA in Vibrio parahaemolyticus.
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副溶血弧菌中群体感应调节因子 AphA 识别的直接靶基因和顺式作用共识的分子特征
DOI：
10.1371/journal.pone.0044210
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhou D
中科院分区：
文献类型：
--
作者：
Sun F;Zhang Y;Wang L;Yan X;Tan Y;Guo Z;Qiu J;Yang R;Xia P;Zhou D
背景AphA是弧菌在低细胞密度下的主要群体感应（QS）调节剂。AphA对靶基因的分子调控已在哈维氏弧菌和霍乱弧菌中得到表征，但在副溶血性弧菌中仍知之甚少。方法/主要发现AphA蛋白在副溶血性弧菌、弧菌Ex 25、弧菌EJY 3、哈维氏弧菌、创伤弧菌、华丽弧菌、鳗弧菌、霍乱弧菌和贝氏弧菌中非常保守。上述9个AphA直系同源物似乎识别保守的顺式作用DNA信号，其可以由两个共有构建体、20 bp盒序列和位置频率矩阵表示。副溶血性弧菌AphA通过直接AphA-靶启动子DNA结合抑制ahpA、qrr 4和opaR的转录，而它以间接方式抑制qrr 2 -3的转录。翻译和转录起始点、σ因子识别的核心启动子元件、核糖体识别的Shine-Dalgarno序列和AphA结合位点（含有相应的AphA盒样序列）在副溶血性弧菌中针对三个直接AphA靶点ahpA、qrr 4和opaR进行了测定。结论/意义AphA介导的ahpA，qrr 2 -4和opaR的抑制在副溶血性弧菌中通过使用多个生化和分子实验来表征。启动子的计算分析表明，保守的机制，转录调控的QS调节器编码基因ahpA，qrr 4，和opaR在弧菌。
Background AphA is the master quorum-sensing (QS) regulator operating at low cell density in vibrios. Molecular regulation of target genes by AphA has been characterized in Vibrio harveyi and V. cholerae, but it is still poorly understood in V. parahaemolyticus. Methodology/Principal Findings The AphA proteins are extremely conserved in V. parahaemolyticus, Vibrio sp. Ex25, Vibrio sp. EJY3, V. harveyi, V. vulnificus, V. splendidus, V. anguillarum, V. cholerae, and V. furnissii. The above nine AphA orthologs appear to recognize conserved cis-acting DNA signals which can be represented by two consensus constructs, a 20 bp box sequence and a position frequency matrix. V. parahaemolyticus AphA represses the transcription of ahpA, qrr4, and opaR through direct AphA-target promoter DNA association, while it inhibits the qrr2-3 transcription in an indirect manner. Translation and transcription starts, core promoter elements for sigma factor recognition, Shine-Dalgarno sequences for ribosome recognition, and AphA-binding sites (containing corresponding AphA box-like sequences) were determined for the three direct AphA targets ahpA, qrr4, and opaR in V. parahaemolyticus. Conclusions/Significance AphA-mediated repression of ahpA, qrr2-4, and opaR was characterized in V. parahaemolyticus by using multiple biochemical and molecular experiments. The computational promoter analysis indicated the conserved mechanism of transcriptional regulation of QS regulator-encoding genes ahpA, qrr4, and opaR in vibrios.
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影响因子：
3.2
作者：
Kovacikova, G;Lin, W;Skorupski, K
通讯作者：
Skorupski, K
影响因子：
3.6
作者：
Lin, Wei;Kovacikova, Gabriela;Skorupski, Karen
通讯作者：
Skorupski, Karen
影响因子：
3.6
作者：
Kovacikova, G;Skorupski, K
通讯作者：
Skorupski, K
影响因子：
5.1
作者：
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通讯作者：
Mazel, Didier
影响因子：
3.2
作者：
Henke, JM;Bassler, BL
通讯作者：
Bassler, BL