A Consistent Protocol Reveals a Large Heterogeneity in the Biological Effectiveness of Proton and Carbon-Ion Beams for Various Sarcoma and Normal-Tissue-Derived Cell Lines.
A Consistent Protocol Reveals a Large Heterogeneity in the Biological Effectiveness of Proton and Carbon-Ion Beams for Various Sarcoma and Normal-Tissue-Derived Cell Lines.
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DOI：
10.3390/cancers14082009
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发表时间：
2022-04-15
期刊：
影响因子：
5.2
通讯作者：
Ogawa, Kazuhiko
中科院分区：
文献类型：
--
作者：
Yagi, Masashi;Takahashi, Yutaka;Minami, Kazumasa;Matsuura, Taeko;Nam, Jin-Min;Onodera, Yasuhito;Akagi, Takashi;Maeda, Takuya;Okimoto, Tomoaki;Shirato, Hiroki;Ogawa, Kazuhiko
使用一致的实验方案，我们发现质子和碳离子束在各种肉瘤和正常组织来源的细胞系中的相对生物有效性(RBE)值存在很大的异质性。我们的数据表明，质子束疗法可能对某些类型的肿瘤更有利。在碳离子治疗中，对于某些类型的肿瘤，RBE的大的异质性应该促使考虑减少剂量或增加每部分的剂量。特别是，正常组织中较高的RBE值需要谨慎。质子和碳离子治疗都需要对肿瘤和正常组织进行特定剂量评估。这项研究调查了不同肉瘤和正常组织来源的细胞系(正常细胞系)在质子和碳离子照射下的相对生物效应(RBE)值的变化。我们使用了一个一致的方案，规定了细胞接种后照射的时间，并详细说明了克隆形成实验。我们研究了四种人肉瘤细胞系(MG63骨肉瘤、HT1080纤维肉瘤、SW872脂肪肉瘤和SW1353软骨肉瘤)和三种正常细胞系(HDF人真皮成纤维细胞、hTERT-HME1乳腺和NuLi-1支气管上皮)对质子束和碳离子照射的RBE的细胞类型依赖性。以54.4keV/μm的线性能量转移用伽马射线、位于展开的布拉格峰中心的质子束或碳离子束照射电池。在所有肉瘤和正常细胞系中，质子和碳离子照射的平均RBE值分别为1.08±0.11和2.08±0.36，分别与目前治疗计划系统中使用的1.1和2.13的值一致。MG63和HT1080的质子束径向效应相差高达34%。同样，在SW872和其他肉瘤细胞系之间也观察到碳离子束的RBE有32%的差异。在质子束照射中，正常细胞系的RBE值变化较小(在10%以内)，而在碳离子照射下，hTERT-HME1和NuLi-1的RBE值相差高达48%。我们的结果表明，在质子和碳离子治疗中，肿瘤和正常组织的特定剂量评估对于治疗计划是必要的。
Using a consistent experimental protocol, we found a large heterogeneity in the relative biological effectiveness (RBE) values of both proton and carbon-ion beams in various sarcomas and normal-tissue-derived cell lines. Our data suggest that proton beam therapy may be more beneficial for some types of tumors. In carbon-ion therapy, for some types of tumors, large heterogeneity in RBE should prompt consideration of dose reduction or an increased dose per fraction. In particular, a higher RBE value in normal tissues requires caution. Specific dose evaluations for tumor and normal tissues are needed for both proton and carbon-ion therapies. This study investigated variations in the relative biological effectiveness (RBE) values among various sarcoma and normal-tissue-derived cell lines (normal cell line) in proton beam and carbon-ion irradiations. We used a consistent protocol that specified the timing of irradiation after plating cells and detailed the colony formation assay. We examined the cell type dependence of RBE for proton beam and carbon-ion irradiations using four human sarcoma cell lines (MG63 osteosarcoma, HT1080 fibrosarcoma, SW872 liposarcoma, and SW1353 chondrosarcoma) and three normal cell lines (HDF human dermal fibroblast, hTERT-HME1 mammary gland, and NuLi-1 bronchus epithelium). The cells were irradiated with gamma rays, proton beams at the center of the spread-out Bragg peak, or carbon-ion beams at 54.4 keV/μm linear energy transfer. In all sarcoma and normal cell lines, the average RBE values in proton beam and carbon-ion irradiations were 1.08 ± 0.11 and 2.08 ± 0.36, which were consistent with the values of 1.1 and 2.13 used in current treatment planning systems, respectively. Up to 34% difference in the RBE of the proton beam was observed between MG63 and HT1080. Similarly, a 32% difference in the RBE of the carbon-ion beam was observed between SW872 and the other sarcoma cell lines. In proton beam irradiation, normal cell lines had less variation in RBE values (within 10%), whereas in carbon-ion irradiation, RBE values differed by up to 48% between hTERT-HME1 and NuLi-1. Our results suggest that specific dose evaluations for tumor and normal tissues are necessary for treatment planning in both proton and carbon-ion therapies.
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影响因子：
5.2
作者：
Marcus D;Lieverse RIY;Klein C;Abdollahi A;Lambin P;Dubois LJ;Yaromina A
通讯作者：
Yaromina A
影响因子：
2.1
作者：
Oliphant, Travis E.
通讯作者：
Oliphant, Travis E.
影响因子：
6.2
作者：
Matsunobu, Akira;Imai, Reiko;Tatezaki, Shin-ichiro
通讯作者：
Tatezaki, Shin-ichiro
影响因子：
2
作者：
Karasawa, Kumiko;Wakatsuki, Masaru;Kamada, Tadashi
通讯作者：
Kamada, Tadashi
影响因子：
3.4
作者：
Cromheecke, M;Piers, BA;Hoekstra, HJ
通讯作者：
Hoekstra, HJ