New insights on the reorganization of gene transcription in Pseudomonas putida KT2440 at elevated pressure.
New insights on the reorganization of gene transcription in Pseudomonas putida KT2440 at elevated pressure.
复制标题
DOI：
10.1186/1475-2859-12-30
复制
发表时间：
2013-03-28
影响因子：
6.4
通讯作者：
Prieto MA
中科院分区：
文献类型：
--
作者：
Follonier S;Escapa IF;Fonseca PM;Henes B;Panke S;Zinn M;Prieto MA
在大型工业生物反应器的底部以及在施加压力以增强氧传递的生物过程期间，容易遇到升高的压力、升高的氧张力（DOT）和升高的二氧化碳张力（DCT）。然而，关于它们对细菌及其基因表达的影响的信息很少。为了阐明受这些特定环境条件影响的细胞功能，使用DNA微阵列彻底研究了恶臭假单胞菌KT 2440（一种与生产中链长度聚羟基链烷酸酯非常相关的细菌）的转录组。用恶臭假单胞菌进行非常明确的恒化器培养，以产生高质量的RNA样品，并确保差异基因表达仅由压力、DOT和/或DCT的变化引起。在7巴和升高的DCT下以热休克和氧化应激样反应的形式检测到细胞应激，并且还鉴定了细胞包膜扰动的指标。总体而言，当DOT在7巴和DCT升高时从40 ± 5%增加到235 ± 20%时，基因转录没有显著改变。然而，与铁硫簇组装、末端氧化酶、谷氨酸代谢和精氨酸脱亚胺酶途径相关的少数基因的转录水平存在差异，表明它们对DOT的变化特别敏感。本研究对恶臭假单胞菌在压力、DOT和DCT轻度变化时转录组的变化进行了全面的综述。有趣的是，虽然基因转录的变化是普遍的，但细胞生理几乎没有受到影响，这说明了基因转录的重组对于处理环境变化是多么有效，否则可能是有害的。几个特别敏感的细胞功能被确定，这肯定会有助于了解的机制，参与应力传感/响应，并找到提高微生物的耐应力的方法。
Elevated pressure, elevated oxygen tension (DOT) and elevated carbon dioxide tension (DCT) are readily encountered at the bottom of large industrial bioreactors and during bioprocesses where pressure is applied for enhancing the oxygen transfer. Yet information about their effect on bacteria and on the gene expression thereof is scarce. To shed light on the cellular functions affected by these specific environmental conditions, the transcriptome of Pseudomonas putida KT2440, a bacterium of great relevance for the production of medium-chain-length polyhydroxyalkanoates, was thoroughly investigated using DNA microarrays. Very well defined chemostat cultivations were carried out with P. putida to produce high quality RNA samples and ensure that differential gene expression was caused exclusively by changes of pressure, DOT and/or DCT. Cellular stress was detected at 7 bar and elevated DCT in the form of heat shock and oxidative stress-like responses, and indicators of cell envelope perturbations were identified as well. Globally, gene transcription was not considerably altered when DOT was increased from 40 ± 5 to 235 ± 20% at 7 bar and elevated DCT. Nevertheless, differential transcription was observed for a few genes linked to iron-sulfur cluster assembly, terminal oxidases, glutamate metabolism and arginine deiminase pathway, which shows their particular sensitivity to variations of DOT. This study provides a comprehensive overview on the changes occurring in the transcriptome of P. putida upon mild variations of pressure, DOT and DCT. Interestingly, whereas the changes of gene transcription were widespread, the cell physiology was hardly affected, which illustrates how efficient reorganization of the gene transcription is for dealing with environmental changes that may otherwise be harmful. Several particularly sensitive cellular functions were identified, which will certainly contribute to the understanding of the mechanisms involved in stress sensing/response and to finding ways of enhancing the stress tolerance of microorganisms.
登录
查看更多内容
影响因子：
14.9
作者：
Davidsen T;Beck E;Ganapathy A;Montgomery R;Zafar N;Yang Q;Madupu R;Goetz P;Galinsky K;White O;Sutton G
通讯作者：
Sutton G
影响因子：
3.6
作者：
Cases, I;deLorenzo, V;PerezMartin, J
通讯作者：
PerezMartin, J
影响因子：
4.9
作者：
CHAMBERLAND, S;BAYER, AS;BRYAN, LE
通讯作者：
BRYAN, LE
影响因子：
3.6
作者：
AUGER, EA;REDDING, KE;BENNETT, GN
通讯作者：
BENNETT, GN
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y