BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons.
BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons.
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DOI：
10.1038/s41467-021-24759-5
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发表时间：
2021-07-26
影响因子：
16.6
通讯作者：
Wiegert JS
中科院分区：
文献类型：
--
作者：
Vierock J;Rodriguez-Rozada S;Dieter A;Pieper F;Sims R;Tenedini F;Bergs ACF;Bendifallah I;Zhou F;Zeitzschel N;Ahlbeck J;Augustin S;Sauter K;Papagiakoumou E;Gottschalk A;Soba P;Emiliani V;Engel AK;Hegemann P;Wiegert JS
通过兴奋性和抑制性视蛋白调控神经元活动的光遗传学研究已成为神经科学研究中不可或缺的实验策略。对于许多应用，需要在单个实验中允许相同神经元的激发和抑制的神经元活动的双向控制。这需要兴奋性和抑制性视蛋白之间的低光谱重叠、匹配的光电流幅度和固定的表达比率。此外，由于所有视蛋白的蓝光敏感性，用不同的光遗传学致动器独立激活两个不同的神经元群体仍然具有挑战性。在这里，我们报告了BiPOLES，一种用两种不同波长的光进行有效神经元激发和抑制的光遗传学工具。BiPOLES能够通过单光子或双光子激发、膜电压的光学调谐以及使用第二蓝光敏感视蛋白对两个神经元群体的独立光遗传学控制实现灵敏、可靠的双色神经元尖峰和沉默。在蠕虫、苍蝇、小鼠和雪貂中证明了BiPOLES的效用。目前，在同一实验中双向控制相同神经元的活动是困难的。在这里，作者报告了一种用于光诱导兴奋和沉默的双向视蛋白对，BiPOLES，他们在一系列生物中使用，包括蠕虫，果蝇，小鼠和雪貂。
Optogenetic manipulation of neuronal activity through excitatory and inhibitory opsins has become an indispensable experimental strategy in neuroscience research. For many applications bidirectional control of neuronal activity allowing both excitation and inhibition of the same neurons in a single experiment is desired. This requires low spectral overlap between the excitatory and inhibitory opsin, matched photocurrent amplitudes and a fixed expression ratio. Moreover, independent activation of two distinct neuronal populations with different optogenetic actuators is still challenging due to blue-light sensitivity of all opsins. Here we report BiPOLES, an optogenetic tool for potent neuronal excitation and inhibition with light of two different wavelengths. BiPOLES enables sensitive, reliable dual-color neuronal spiking and silencing with single- or two-photon excitation, optical tuning of the membrane voltage, and independent optogenetic control of two neuronal populations using a second, blue-light sensitive opsin. The utility of BiPOLES is demonstrated in worms, flies, mice and ferrets. Currently, bidirectional control of activity in the same neurons in the same experiment is difficult. Here the authors report a Bidirectional Pair of Opsins for Light-induced Excitation and Silencing, BiPOLES, which they use in a range of organisms including worms, fruit flies, mice and ferrets.
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影响因子：
64.8
作者：
Kim YS;Kato HE;Yamashita K;Ito S;Inoue K;Ramakrishnan C;Fenno LE;Evans KE;Paggi JM;Dror RO;Kandori H;Kobilka BK;Deisseroth K
通讯作者：
Deisseroth K
影响因子：
11.4
作者：
FIRE, A
通讯作者：
FIRE, A
影响因子：
--
作者：
Gee, Christine E;Ohmert, Iris;Oertner, Thomas G
通讯作者：
Oertner, Thomas G
影响因子：
7.7
作者：
Baker, Christopher A.;Elyadat, Yishai M.;Bolton, M. McLean
通讯作者：
Bolton, M. McLean
影响因子：
4.6
作者：
Heikenfeld, Carla;Mederos, Sara;Ponomarenko, Alexey
通讯作者：
Ponomarenko, Alexey