Conversion of the Pseudomonas aeruginosa Quinolone Signal and Related Alkylhydroxyquinolines by Rhodococcus sp. Strain BG43
Conversion of the Pseudomonas aeruginosa Quinolone Signal and Related Alkylhydroxyquinolines by Rhodococcus sp. Strain BG43
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红球菌菌株 BG43 对铜绿假单胞菌喹诺酮信号和相关烷基羟基喹啉的转化
DOI：
10.1128/aem.02342-14
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发表时间：
2014
影响因子：
4.4
通讯作者：
Fetzner
中科院分区：
文献类型：
--
作者：
Müller;Birmes;Niewerth;Fetzner
利用喹诺酮假单胞菌（Pseudomonas quinolone signal， PQS）从土壤中分离到一株与红polis红球菌密切相关的红球菌（Rhodococcus sp.）。2-庚基-3-羟基-4(1H)-喹诺酮]，机会致病菌铜绿假单胞菌使用的群体感应信号。该分离物被命名为红球菌属菌株BG43，它通过代谢将PQS及其生物合成前体2-庚基-4(1H)-喹诺酮（HHQ）降解为邻氨基苯甲酸。HHQ降解伴随着PQS的瞬时生成，细胞提取物对HHQ的羟基化需要NADH，说明菌株BG43具有与铜绿假单胞菌生物合成酶PqsH同工的HHQ单加氧酶。催化HHQ羟基化和PQS降解的酶均可被PQS诱导，表明其具有特异性途径。值得注意的是，Rhodococcus sp. BG43也能够将2-庚基-4-羟基喹啉- n -氧化物转化为PQS。因此，它将铜绿假单胞菌的抗菌次级代谢物转化为群体感应信号分子。
A bacterial strain, which based on the sequences of its 16S rRNA,gyrB,catA, andqsdAgenes, was identified as a Rhodococcus sp. closely related to Rhodococcus erythropolis, was isolated from soil by enrichment on the Pseudomonas quinolone signal [PQS; 2-heptyl-3-hydroxy-4(1H)-quinolone], a quorum sensing signal employed by the opportunistic pathogen Pseudomonas aeruginosa. The isolate, termed Rhodococcus sp. strain BG43, cometabolically degraded PQS and its biosynthetic precursor 2-heptyl-4(1H)-quinolone (HHQ) to anthranilic acid. HHQ degradation was accompanied by transient formation of PQS, and HHQ hydroxylation by cell extracts required NADH, indicating that strain BG43 has a HHQ monooxygenase isofunctional to the biosynthetic enzyme PqsH of P. aeruginosa. The enzymes catalyzing HHQ hydroxylation and PQS degradation were inducible by PQS, suggesting a specific pathway. Remarkably, Rhodococcus sp. BG43 is also capable of transforming 2-heptyl-4-hydroxyquinoline-N-oxide to PQS. It thus converts an antibacterial secondary metabolite of P. aeruginosa to a quorum sensing signal molecule.
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