Increased extracellular matrix stiffness accompanies compromised bladder function in a murine model of radiation cystitis.
Increased extracellular matrix stiffness accompanies compromised bladder function in a murine model of radiation cystitis.
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DOI：
10.1016/j.actbio.2022.03.017
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发表时间：
2022-05
影响因子：
9.7
通讯作者：
Roccabianca, Sara
中科院分区：
文献类型：
--
作者：
Zwaans, Bernadette M. M.;Grobbel, Marissa;Carabulea, Alexander L.;Lamb, Laura E.;Roccabianca, Sara
放射性膀胱炎是一种由于盆腔放射治疗引起的长期膀胱缺损，可导致下尿路症状，如尿频和排尿困难，提示膀胱顺应性受损。本研究的目的是确定放射性膀胱炎小鼠模型的膀胱细胞外基质的机械行为的改变，在辐射暴露后3个月和6个月。本研究的结果表明，与年龄匹配的对照组相比，辐照膀胱的细胞外基质的扩张性显著降低。这些发现与功能性膀胱变化一致，包括排尿次数增加和排尿量减少。机械和功能变化在照射后3个月明显，在6个月时具有统计学显著性，表明放射性膀胱炎的进行性。总体而言，本研究的结果表明，照射暴露改变膀胱的机械和生理特性。在人类中，放射性膀胱炎导致下尿路症状，如尿频和尿失禁，提示膀胱顺应性受损。这种病理学可以显着影响癌症幸存者的恢复和生活质量。了解膀胱细胞外基质的力学行为改变如何影响泌尿功能，将对这一人群产生重大影响。本研究的结果表明，在放射性膀胱炎的小鼠模型中，与年龄匹配的对照组相比，照射膀胱的细胞外基质的扩张性显著降低。这些发现伴随着功能性排尿变化，包括排尿次数增加和排尿量减少。本研究的结果揭示了辐射暴露改变了膀胱的机械和生理特性。
Radiation cystitis, a long-term bladder defect due to pelvic radiation therapy, results in lower urinary tract symptoms, such as urinary frequency and nocturia, suggestive of compromised bladder compliance. The goal of this study was to identify alterations to the mechanical behavior of the urinary bladder extracellular matrix of a murine model of radiation cystitis, at 3 and 6 months after radiation exposure. The results of this study demonstrated that the extracellular matrix of irradiated bladders was significantly less distensible when compared to age matching controls. These findings coincided with functional bladder changes, including increased number of voids and decreased voided volume. Both mechanical and functional changes were apparent at 3 months post-irradiation and were statistically significant at 6 months, demonstrating the progressive nature of radiation cystitis. Overall, the results of this study indicate that irradiation exposure changes both the mechanical and physiological properties of the bladder. In humans, radiation cystitis results in lower urinary tract symptoms, such as urinary frequency and nocturia, suggestive of compromised bladder compliance. This pathology can significantly affect recovery and quality of life for cancer survivors. Gaining knowledge about how alterations to the mechanical behavior of the urinary bladder extracellular matrix can affect urinary function will have a significant impact on this population. The results of this study demonstrated that the extracellular matrix of irradiated bladders was significantly less distensible when compared to age matching controls, in a mouse model of radiation cystitis. These findings were accompanied by functional voiding changes, including increased number of voids and decreased voided volume. The results of this study uncovered that irradiation exposure changes the mechanical and physiological properties of the bladder.
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影响因子：
3.5
作者：
Grobbel MR;Shavik SM;Darios E;Watts SW;Lee LC;Roccabianca S
通讯作者：
Roccabianca S
影响因子：
2.1
作者：
Martin, Sarah E.;Begun, Evan M.;Abdelhady, Mazen
通讯作者：
Abdelhady, Mazen
影响因子：
2
作者：
Ikeda, Youko;Zabbarova, Irina V.;Kanai, Anthony J.
通讯作者：
Kanai, Anthony J.
影响因子：
2.4
作者：
Natali, A. N.;Audenino, A. L.;Zanetti, E. M.
通讯作者：
Zanetti, E. M.
影响因子：
3.5
作者：
Cheng F;Birder LA;Kullmann FA;Hornsby J;Watton PN;Watkins S;Thompson M;Robertson AM
通讯作者：
Robertson AM