Layer-dependent role of collagen recruitment during loading of the rat bladder wall.
Layer-dependent role of collagen recruitment during loading of the rat bladder wall.
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DOI：
10.1007/s10237-017-0968-5
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发表时间：
2018-04
影响因子：
3.5
通讯作者：
Robertson AM
中科院分区：
文献类型：
--
作者：
Cheng F;Birder LA;Kullmann FA;Hornsby J;Watton PN;Watkins S;Thompson M;Robertson AM
在这项工作中，我们重新评估了长期以来关于膀胱壁异常大顺应性的来源的猜测。鉴于这些推测是基于对加载机制的间接测量，在这项工作中，我们利用生物成像的进展来直接评估双向加载过程中的胶原纤维和壁结构。使用与多光子显微镜(MPM)兼容的定制双轴力学测试系统，直接测量了9只雄性Fischer大鼠(4只成年和5只老年)膀胱组织中层依赖性胶原纤维的募集。对于其他软组织，膀胱负荷曲线呈指数型，可分为趾区、过渡区和高应力区。在内层(固有层)和外层(逼尿肌平滑肌层)的背景下，评估胶原募集和负荷曲线之间的关系。膀胱的巨大延展性被发现是由于壁上的褶皱(皱纹)提供了一种低阻平化的机制，而在整个脚趾区域没有任何明显的胶原纤维重新聚集。对于更具伸展性的膀胱，随着负荷扩展到过渡阶段，固有层(LP)和逼尿肌平滑层(DSM)之间的胶原纤维逐渐协调募集。第二个重要的发现是，外壁的胶原蛋白过早地重新聚集，从而缩短了脚趾区域，可能会失去管壁的延展性。这种变化与年龄有关。这项工作首次为胶原蛋白在决定膀胱延展性和容量中的作用提供了一个机械性的理解。
In this work, we reevaluated long standing conjectures as to the source of the exceptionally large compliance of the bladder wall. Whereas, these conjectures were based on indirect measures of loading mechanisms, in this work we take advantage of advances in bioimaging to directly assess collagen fibers and wall architecture during biaxial loading. A custom biaxial mechanical testing system compatible with multiphoton microscopy (MPM) was used to directly measure the layer dependent collagen fiber recruitment in bladder tissue from 9 male Fischer rats (4 adult and 5 aged). As for other soft tissues, the bladder loading curve was exponential in shape and could be divided into toe, transition and high stress regimes. The relationship between collagen recruitment and loading curves was evaluated in the context of the inner (lamina propria) and outer (detrusor smooth muscle) layers. The large extensibility of the bladder was found to be possible due to folds in the wall (rugae) that provide a mechanism for low resistance flattening without any discernible recruitment of collagen fibers throughout the toe regime. For more extensible bladders, as the loading extended into the transition regime, a gradual coordinated recruitment of collagen fibers between the lamina propria layer (LP) and detrusor smooth muscle layer (DSM) was found. A second important finding was that wall extensibility could be lost by premature recruitment of collagen in the outer wall that cut short the toe region. This change was correlated with age. This work provides, for the first time, a mechanistic understanding of the role of collagen recruitment in determining bladder extensibility and capacitance.
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6.6
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