Pro-Inflammatory Alterations of Circulating Monocytes in Latent Tuberculosis Infection.
Pro-Inflammatory Alterations of Circulating Monocytes in Latent Tuberculosis Infection.
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DOI：
10.1093/ofid/ofac629
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发表时间：
2022-12
影响因子：
4.2
通讯作者：
中科院分区：
文献类型：
--
作者：
潜伏结核感染（LTBI）与心血管风险增加有关。我们研究了LTBI患者中单核细胞的激活和促炎特征及其与冠状动脉疾病（CAD）的关系。在秘鲁利马，40-70岁的个体接受了QuantiFERON-TB测试来确定LTBI，完成了冠状动脉计算机断层血管造影来评估CAD，并提供了血液用于流式细胞术的单核细胞分析。用脂多糖刺激细胞以评估白细胞介素-6 （IL-6）和肿瘤坏死因子(TNF) -α的反应。LTBI组（n = 28）和非LTBI组（n = 41）的临床特征相似。与非LTBI个体相比，LTBI个体的所有单核细胞亚群均表现出更高的CX3CR1和CD36的平均荧光强度（MFI）。LTBI患者表达IL-6 （44.9 vs 26.9, P = 0.014）、TNF-α (62.3 vs 35.1, P = 0.014)和TNF-α+IL-6+ (43.2 vs 36.6, P = 0.042)的非经典单核细胞比例增加。在LTBI个体中，CAD与经典单核细胞CX3CR1 MFI降低和所有单核细胞亚群CD36 MFI降低相关。在多变量分析中，总单核细胞CD36 MFI的降低（b = - 0.17; P = 0.002）和所有亚群仍然与LTBI中的CAD独立相关。LTBI患者有明显的单核细胞改变，提示炎症反应和组织迁移加剧。这些改变是否有助于心血管疾病的发病机制有待进一步研究。
Latent tuberculosis infection (LTBI) has been associated with increased cardiovascular risk. We investigated the activation and pro-inflammatory profile of monocytes in individuals with LTBI and their association with coronary artery disease (CAD). Individuals 40–70 years old in Lima, Peru, underwent QuantiFERON-TB testing to define LTBI, completed a coronary computed tomography angiography to evaluate CAD, and provided blood for monocyte profiling using flow cytometry. Cells were stimulated with lipopolysaccharide to assess interleukin-6 (IL-6) and tumor necrosis factor (TNF)–α responses. The clinical characteristics of the LTBI (n = 28) and non-LTBI (n = 41) groups were similar. All monocyte subsets from LTBI individuals exhibited higher mean fluorescence intensity (MFI) of CX3CR1 and CD36 compared with non-LTBI individuals. LTBI individuals had an increased proportion of nonclassical monocytes expressing IL-6 (44.9 vs 26.9; P = .014), TNF-α (62.3 vs 35.1; P = .014), and TNF-α+IL-6+ (43.2 vs 36.6; P = .042). Among LTBI individuals, CAD was associated with lower CX3CR1 MFI on classical monocytes and lower CD36 MFI across all monocyte subsets. In multivariable analyses, lower CD36 MFI on total monocytes (b = −0.17; P = .002) and all subsets remained independently associated with CAD in LTBI. Individuals with LTBI have distinct monocyte alterations suggestive of an exacerbated inflammatory response and tissue migration. Whether these alterations contribute to cardiovascular disease pathogenesis warrants further investigation.
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影响因子：
13.8
作者：
Hoffmann U;Lu MT;Foldyna B;Zanni MV;Karady J;Taron J;Zhai BK;Burdo T;Fitch KV;Kileel EM;Williams K;Fichtenbaum CJ;Overton ET;Malvestutto C;Aberg J;Currier J;Sponseller CA;Melbourne K;Floris-Moore M;Van Dam C;Keefer MC;Koletar SL;Douglas PS;Ribaudo H;Mayrhofer T;Grinspoon SK;REPRIEVE trial
通讯作者：
REPRIEVE trial
DOI：
10.11604/pamj.2021.38.362.28110
发表时间：
2021
期刊：
The Pan African medical journal
影响因子：
--
作者：
Khoufi EAA
通讯作者：
Khoufi EAA
影响因子：
3.2
作者：
Castano, Diana;Garcia, Luis F.;Rojas, Mauricio
通讯作者：
Rojas, Mauricio
影响因子：
4.6
作者：
Hunt, Peter W.
通讯作者：
Hunt, Peter W.
影响因子：
3.9
作者：
Guo, Na;Chen, Yongchang;Zhang, Tong
通讯作者：
Zhang, Tong