Simultaneous co‐overexpression of Saccharomyces cerevisiae septins Cdc3 and Cdc10 drives pervasive, phospholipid‐, and tag‐dependent plasma membrane localization
Simultaneous co‐overexpression of Saccharomyces cerevisiae septins Cdc3 and Cdc10 drives pervasive, phospholipid‐, and tag‐dependent plasma membrane localization
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酿酒酵母脓毒症 Cdc3 和 Cdc10 的同时共过表达驱动普遍的、磷脂和标签依赖性质膜定位
DOI：
10.1002/cm.21762
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发表时间：
2023
期刊：
影响因子：
2.9
通讯作者：
McMurray, Michael
中科院分区：
文献类型：
--
作者：
Benson, Aleyna;McMurray, Michael
Septin蛋白有助于许多涉及细胞膜的真核生物过程。在芽殖酵母酿酒酵母中，septin异源寡聚体几乎完全在胞质分裂的未来位点与质膜（PM）相互作用。虽然已经确定了膜募集的多种机制，包括与特定磷脂的直接相互作用和通过两亲性螺旋的曲率敏感性相互作用，但这些并不能完全解释为什么酵母胞苷不能定位在PM的整个内小叶上。在设计诱导型分裂黄色荧光蛋白（YFP）系统以测量酵母septin复合物组装的动力学时，我们发现两种标记septin Cdc 3和Cdc 10的异位共过表达导致几乎均匀的PM定位，以及内源性septin功能的扰动。隔膜定位和功能的配子发生也受到干扰。PM定位需要与Cdc 3的C末端融合的C末端YFP片段、隔蛋白相关激酶Cla 4和Gin 4以及磷脂酰肌醇-4，5-二磷酸（PI[4，5]P2），但不需要Cdc 3中推定的PI（4，5）P2结合残基。内源性Cdc 10被募集到PM，可能有助于功能干扰。PM定位的胞隔膜不与细胞溶质池交换，表明聚合物稳定。这些发现提供了新的线索，通常是什么限制septin定位到特定的膜。
Septin proteins contribute to many eukaryotic processes involving cellular membranes. In the budding yeastSaccharomyces cerevisiae, septin hetero‐oligomers interact with the plasma membrane (PM) almost exclusively at the future site of cytokinesis. While multiple mechanisms of membrane recruitment have been identified, including direct interactions with specific phospholipids and curvature‐sensitive interactions via amphipathic helices, these do not fully explain why yeast septins do not localize all over the inner leaflet of the PM. While engineering an inducible split‐yellow fluorescent protein (YFP) system to measure the kinetics of yeast septin complex assembly, we found that ectopic co‐overexpression of two tagged septins, Cdc3 and Cdc10, resulted in nearly uniform PM localization, as well as perturbation of endogenous septin function. Septin localization and function in gametogenesis were also perturbed. PM localization required the C‐terminal YFP fragment fused to the C terminus of Cdc3, the septin‐associated kinases Cla4 and Gin4, and phosphotidylinositol‐4,5‐bis‐phosphate (PI[4,5]P2), but not the putative PI(4,5)P2‐binding residues in Cdc3. Endogenous Cdc10 was recruited to the PM, likely contributing to the functional interference. PM‐localized septins did not exchange with the cytosolic pool, indicative of stable polymers. These findings provide new clues as to what normally restricts septin localization to specific membranes.
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影响因子：
4.1
作者：
Rodríguez-Escudero, I;Roelants, FM;Cid, VJ
通讯作者：
Cid, VJ
影响因子：
3.3
作者：
Versele, M;Gullbrand, B;Thorner, J
通讯作者：
Thorner, J
septins 检测膜曲率的长短
DOI：
10.1083/jcb.201903045
发表时间：
2019
期刊：
The Journal of Cell Biology
影响因子：
--
作者：
M. McMurray
通讯作者：
M. McMurray
影响因子：
5.6
作者：
Bertin A;McMurray MA;Thai L;Garcia G 3rd;Votin V;Grob P;Allyn T;Thorner J;Nogales E
通讯作者：
Nogales E
DOI：
--
发表时间：
2007
期刊：
--
影响因子：
--
作者：
Fatima Cvr;E. Manser;J. Pringle
通讯作者：
Fatima Cvr;E. Manser;J. Pringle