Identification of salivary mucin MUC7 binding proteins from Streptococcus gordonii.
Identification of salivary mucin MUC7 binding proteins from Streptococcus gordonii.
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DOI：
10.1186/1471-2180-9-163
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发表时间：
2009-08-11
期刊：
影响因子：
4.2
通讯作者：
Sheehan JK
中科院分区：
文献类型：
--
作者：
Kesimer M;Kiliç N;Mehrotra R;Thornton DJ;Sheehan JK
唾液粘蛋白 MUC7（以前称为 MG2）可以粘附各种链球菌菌株，这些链球菌菌株是口腔的主要定殖者和主要微生物。尽管人们对口腔病原体和唾液粘蛋白之间的相互作用越来越感兴趣，但报告细菌特异性结合位点的研究相当有限。细菌细胞表面特定相互作用蛋白（称为粘附素）的鉴定和表征对于进一步了解宿主与病原体的相互作用至关重要。我们在此证明，使用纯化的 MUC7 覆盖格氏链球菌细胞表面蛋白 SDS 提取物的印迹，4 个 MUC7 结合带，来自格氏链球菌菌株 PK488 的表观分子质量为 62、78、84 和 133 kDa。通过凝胶内消化和随后对所得肽进行 NanoLC 串联质谱分析来鉴定推定的粘附素。 62 kDa 和 84 kDa 条带分别被鉴定为延伸因子 (EF) Tu 和 EF-G。 78 kDa条带是hppA基因产物； 74 kDa 寡肽结合脂蛋白。 133 kDa 条带包含两种蛋白质； α 烯醇酶和 DNA 指导的 RNA 聚合酶，β' 亚基。其中一些蛋白质，例如α烯醇化酶，预计位于细胞内，然而，流式细胞术分析证实了其在细菌表面的位置。我们的数据表明，S. gordonii 表达了许多假定的 MUC7 识别蛋白，这些蛋白有助于该链球菌菌株的 MUC7 粘蛋白结合。
The salivary mucin MUC7 (previously known as MG2) can adhere to various strains of streptococci that are primary colonizers and predominant microorganisms of the oral cavity. Although there is a growing interest in interaction between oral pathogens and salivary mucins, studies reporting the specific binding sites on the bacteria are rather limited. Identification and characterization of the specific interacting proteins on the bacterial cell surface, termed adhesins, are crucial to further understand host-pathogen interactions. We demonstrate here, using purified MUC7 to overlay blots of SDS-extracts of Streptococcus gordonii cell surface proteins, 4 MUC7-binding bands, with apparent molecular masses of 62, 78, 84 and 133 kDa from the Streptococcus gordonii strain, PK488. Putative adhesins were identified by in-gel digestion and subsequent nanoLC-tandem mass spectrometry analysis of resultant peptides. The 62 kDa and 84 kDa bands were identified as elongation factor (EF) Tu and EF-G respectively. The 78 kDa band was a hppA gene product; the 74 kDa oligopeptide-binding lipoprotein. The 133 kDa band contained two proteins; alpha enolase and DNA-directed RNA polymerase, beta' subunit. Some of these proteins, for example alpha enolase are expected to be intracellular, however, flow cytometric analysis confirmed its location on the bacterial surface. Our data demonstrated that S. gordonii expressed a number of putative MUC7 recognizing proteins and these contribute to MUC7 mucin binding of this streptococcal strain.
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影响因子：
5.7
作者：
KJELDGAARD, M;NISSEN, P;NYBORG, J
通讯作者：
NYBORG, J
影响因子：
3
作者：
ALHASHIMI, I;LEVINE, MJ
通讯作者：
LEVINE, MJ
影响因子：
3.4
作者：
HANSKI, C;BORNHOEFT, G;RIECKEN, EO
通讯作者：
RIECKEN, EO
影响因子：
3.1
作者：
JENKINSON, HF;TERRY, SD;TANNOCK, GW
通讯作者：
TANNOCK, GW
影响因子：
3.1
作者：
KOLENBRANDER, PE;ANDERSEN, RN
通讯作者：
ANDERSEN, RN