Chromosomal inversions and ecotypic differentiation in Anopheles gambiae: the perspective from whole-genome sequencing.
Chromosomal inversions and ecotypic differentiation in Anopheles gambiae: the perspective from whole-genome sequencing.
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DOI：
10.1111/mec.13888
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发表时间：
2016-12
影响因子：
4.9
通讯作者：
Besansky NJ
中科院分区：
文献类型：
--
作者：
Love RR;Steele AM;Coulibaly MB;Traore SF;Emrich SJ;Fontaine MC;Besansky NJ
促进谱系适应性辐射的分子机制和遗传结构仍然难以捉摸。多态性染色体倒位，由于其重组减少的效果，建议生态分化的工具。在这里，我们研究一个生态多样化的血统。冈比亚，称为巴马科染色体形式的基础上，其独特的互补的三个染色体倒位，探讨这些倒位对生态型分化的影响。我们使用了巴马科、典型（非巴马科）An. gambiae和姐妹种An. coluzzii研究进化关系和全基因组的核苷酸多样性和谱系之间的分化模式。尽管广泛的共享多态性和有限的分化，从其他类群，巴马科集群除了其他类群，并形成了最大限度地支持分支的邻居加入树的基础上，全基因组数据（包括倒位）或仅在共线区域。然而，FST离群值分析表明，巴马科和典型的An之间的大部分分化区域。冈比亚位于染色体倒位内，与它们在巴马科生态隔离中的作用一致。异常分化的基因组区域富含与神经系统发育和信号传导有关的基因。候选基因与选择性扫描独特的巴马科包含未观察到的其他类群的同域样本中的取代，和几个杀虫剂抗性基因等位基因之间共享巴马科和其他类群分离在这些样品中的频率急剧不同。巴马科是了解这一重要疟疾病媒群体同域种群生态和基因组分化初始阶段的一个有用窗口。
The molecular mechanisms and genetic architecture that facilitate adaptive radiation of lineages remain elusive. Polymorphic chromosomal inversions, due to their recombination-reducing effect, are proposed instruments of ecotypic differentiation. Here we study an ecologically diversifying lineage of An. gambiae, known as the Bamako chromosomal form based on its unique complement of three chromosomal inversions, to explore the impact of these inversions on ecotypic differentiation. We used pooled and individual genome sequencing of Bamako, typical (non-Bamako) An. gambiae, and the sister species An. coluzzii to investigate evolutionary relationships and genome-wide patterns of nucleotide diversity and differentiation among lineages. Despite extensive shared polymorphism and limited differentiation from the other taxa, Bamako clusters apart from the other taxa, and forms a maximally supported clade in neighbor-joining trees based on whole genome data (including inversions) or solely on collinear regions. Nevertheless, FST outlier analysis reveals that the majority of differentiated regions between Bamako and typical An. gambiae are located inside chromosomal inversions, consistent with their role in the ecological isolation of Bamako. Exceptionally differentiated genomic regions were enriched for genes implicated in nervous system development and signaling. Candidate genes associated with a selective sweep unique to Bamako contain substitutions not observed in sympatric samples of the other taxa, and several insecticide resistance gene alleles shared between Bamako and other taxa segregate at sharply different frequencies in these samples. Bamako represents a useful window into the initial stages of ecological and genomic differentiation from sympatric populations in this important group of malaria vectors.
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影响因子：
4.5
作者：
Aboagye-Antwi F;Alhafez N;Weedall GD;Brothwood J;Kandola S;Paton D;Fofana A;Olohan L;Betancourth MP;Ekechukwu NE;Baeshen R;Traorè SF;Diabate A;Tripet F
通讯作者：
Tripet F
影响因子：
--
作者：
Besansky, NJ;Collins, FH;Townson, H
通讯作者：
Townson, H
影响因子：
3.8
作者：
della Torre, A;Tu, ZJ;Petrarca, V
通讯作者：
Petrarca, V
使用下一代 DNA 测序数据进行变异发现和基因分型的框架。
影响因子：
30.8
作者：
通讯作者：
--
影响因子：
4.4
作者：
Cohuet A;Krishnakumar S;Simard F;Morlais I;Koutsos A;Fontenille D;Mindrinos M;Kafatos FC
通讯作者：
Kafatos FC